Slit1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot using Slit1 polyclonal antibody (Cat # PAB11326) shows detection of Slit1 in rat (Lane 1) and mouse (Lane 2) brain lysates.
The expected molecular weight for Slit1 is 168 KDa.
Approximately 20 &micr;g of each lysates was run on a SDS-PAGE and reaction with a 1 : 500 dilution of Slit1 polyclonal antibody.
Note: The smaller strong bands observed in this blot are likely Slit1 cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40 KDa -160 KDa (see Little et al, 2002 for additional details).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Slit1.
Immunogen
A synthetic peptide corresponding to amino acids 487-504 of mouse Slit1.
Host
Rabbit
Reactivity
Chicken, Frog, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using Slit1 polyclonal antibody (Cat # PAB11326) shows detection of Slit1 in rat (Lane 1) and mouse (Lane 2) brain lysates.
The expected molecular weight for Slit1 is 168 KDa.
Approximately 20 &micr;g of each lysates was run on a SDS-PAGE and reaction with a 1 : 500 dilution of Slit1 polyclonal antibody.
Note: The smaller strong bands observed in this blot are likely Slit1 cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40 KDa -160 KDa (see Little et al, 2002 for additional details).Enzyme-linked Immunoabsorbent Assay
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Gene Info — Slit1
Entrez GeneID
20562Protein Accession#
Q80TR4;NP_056563Gene Name
Slit1
Gene Alias
Slil1, mKIAA0813
Gene Description
slit homolog 1 (Drosophila)
Gene Ontology
HyperlinkOther Designations
slit homolog 1
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Publication Reference
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Expression of Developmentally Important Axon Guidance Cues in the Adult Optic Chiasm.
Conceição R, Evans RS, Pearson CS, Hänzi B, Osborne A, Deshpande SS, Martin KR, Barber AC.
Investigative Ophthalmology & Visual Science 2019 Nov; 60(14):4727.
Application：IHC-Fr, Mouse, Mouse brains.
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Conserved roles for Slit and Robo proteins in midline commissural axon guidance.
Long H, Sabatier C, Ma L, Plump A, Yuan W, Ornitz DM, Tamada A, Murakami F, Goodman CS, Tessier-Lavigne M.
Neuron 2004 Apr; 42(2):213.
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Multiple roles for slits in the control of cell migration in the rostral migratory stream.
Nguyen-Ba-Charvet KT, Picard-Riera N, Tessier-Lavigne M, Baron-Van Evercooren A, Sotelo C, Chédotal A.
Journal of Neuroscience 2004 Feb; 24(6):1497.
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On the topographic targeting of basal vomeronasal axons through Slit-mediated chemorepulsion.
Knöll B, Schmidt H, Andrews W, Guthrie S, Pini A, Sundaresan V, Drescher U.
Development 2003 Nov; 130(21):5073.
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Expression of Developmentally Important Axon Guidance Cues in the Adult Optic Chiasm.
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