Western blot using Slit1 polyclonal antibody (Cat # PAB11326) shows detection of Slit1 in rat (Lane 1) and mouse (Lane 2) brain lysates. The expected molecular weight for Slit1 is 168 KDa. Approximately 20 &micr;g of each lysates was run on a SDS-PAGE and reaction with a 1 : 500 dilution of Slit1 polyclonal antibody. Note: The smaller strong bands observed in this blot are likely Slit1 cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40 KDa -160 KDa (see Little et al, 2002 for additional details).