Western blot using Sipa1 polyclonal antibody (Cat # PAB11325) shows detection of over-expressed Sipa1 in lysates from mouse NIH/3T3 cells transfected with Sipa1 (Lane 1). Endogenous Sipa1 is detected in lane 2, which contains lysate from NIH/3T3 cells mock-transfected with Lac ZGLB, although at a significantly reduced level compared to transfected cells. Lane 3 and 4 are similar to lanes 1 and 2 except the antibody was preincubated with the immunizing peptide prior to reaction with the membrane. The identity of the higher and lower molecular weight bands is unknown. The band at ~130 KDa, indicated by the arrowhead, corresponds to recombinant Sipa1. The primary antibody was used at 1 : 1250. Personal communication, H. Yang, L. Lukes and K. Hunter, NCI, Bethesda, MD.
Immunohistochemistry of Sipa1 polyclonal antibody (Cat # PAB11325) was used at 1.25 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate to strong positive staining of lymphocytes within human tonsil at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.