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Last updated: 2017/12/17

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Sipa1 polyclonal antibody

  • Catalog # : PAB11325
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Sipa1.
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus of mouse Sipa1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody is specific to mouse Sipa1 protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:20000)
    Western Blot (1:1000-1:5000)
    Immunohistochemistry (1.25-2.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using Sipa1 polyclonal antibody (Cat # PAB11325) shows detection of over-expressed Sipa1 in lysates from mouse NIH/3T3 cells transfected with Sipa1 (Lane 1).
    Endogenous Sipa1 is detected in lane 2, which contains lysate from NIH/3T3 cells mock-transfected with Lac ZGLB, although at a significantly reduced level compared to transfected cells.
    Lane 3 and 4 are similar to lanes 1 and 2 except the antibody was preincubated with the immunizing peptide prior to reaction with the membrane.
    The identity of the higher and lower molecular weight bands is unknown.
    The band at ~130 KDa, indicated by the arrowhead, corresponds to recombinant Sipa1.
    The primary antibody was used at 1 : 1250.
    Personal communication, H. Yang, L. Lukes and K. Hunter, NCI, Bethesda, MD.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of Sipa1 polyclonal antibody (Cat # PAB11325) was used at 1.25 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
    This image shows moderate to strong positive staining of lymphocytes within human tonsil at 40X.
    Tissue was formalin-fixed and paraffin embedded.
    The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
    Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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  • Gene Information
  • Protein Accession#:
  • NP_035509;P46062
  • Gene Name:
  • Sipa1
  • Gene Alias:
  • KIAA4074,SPA-1,mKIAA4074
  • Gene Description:
  • signal-induced proliferation associated gene 1
  • Other Designations:
  • -
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