Sipa1 polyclonal antibody
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More Files
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Western Blot
Western blot using Sipa1 polyclonal antibody (Cat # PAB11325) shows detection of over-expressed Sipa1 in lysates from mouse NIH/3T3 cells transfected with Sipa1 (Lane 1).
Endogenous Sipa1 is detected in lane 2, which contains lysate from NIH/3T3 cells mock-transfected with Lac ZGLB, although at a significantly reduced level compared to transfected cells.
Lane 3 and 4 are similar to lanes 1 and 2 except the antibody was preincubated with the immunizing peptide prior to reaction with the membrane.
The identity of the higher and lower molecular weight bands is unknown.
The band at ~130 KDa, indicated by the arrowhead, corresponds to recombinant Sipa1.
The primary antibody was used at 1 : 1250.
Personal communication, H. Yang, L. Lukes and K. Hunter, NCI, Bethesda, MD.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Sipa1 polyclonal antibody (Cat # PAB11325) was used at 1.25 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate to strong positive staining of lymphocytes within human tonsil at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Sipa1.
Immunogen
A synthetic peptide corresponding to N-terminus of mouse Sipa1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to mouse Sipa1 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:20000)
Western Blot (1:1000-1:5000)
Immunohistochemistry (1.25-2.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using Sipa1 polyclonal antibody (Cat # PAB11325) shows detection of over-expressed Sipa1 in lysates from mouse NIH/3T3 cells transfected with Sipa1 (Lane 1).
Endogenous Sipa1 is detected in lane 2, which contains lysate from NIH/3T3 cells mock-transfected with Lac ZGLB, although at a significantly reduced level compared to transfected cells.
Lane 3 and 4 are similar to lanes 1 and 2 except the antibody was preincubated with the immunizing peptide prior to reaction with the membrane.
The identity of the higher and lower molecular weight bands is unknown.
The band at ~130 KDa, indicated by the arrowhead, corresponds to recombinant Sipa1.
The primary antibody was used at 1 : 1250.
Personal communication, H. Yang, L. Lukes and K. Hunter, NCI, Bethesda, MD.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Sipa1 polyclonal antibody (Cat # PAB11325) was used at 1.25 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate to strong positive staining of lymphocytes within human tonsil at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Sipa1
Entrez GeneID
20469Protein Accession#
NP_035509;P46062Gene Name
Sipa1
Gene Alias
KIAA4074, SPA-1, mKIAA4074
Gene Description
signal-induced proliferation associated gene 1
Gene Ontology
HyperlinkOther Designations
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Publication Reference
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Bromodomain protein Brd4 binds to GTPase-activating SPA-1, modulating its activity and subcellular localization.
Farina A, Hattori M, Qin J, Nakatani Y, Minato N, Ozato K.
Molecular and Cellular Biology 2004 Oct; 24(20):9059.
Application：WB, Human, Mouse , HeLa, NIH 3T3 cells.
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Rap1 GTPase-activating protein SPA-1 negatively regulates cell adhesion.
Tsukamoto N, Hattori M, Yang H, Bos JL, Minato N.
The Journal of Biological Chemistry 1999 Jun; 274(26):18463.
Application：IF, WB-Tr, Human, Mouse, 32D, HeLa cells.
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Human SPA-1 gene product selectively expressed in lymphoid tissues is a specific GTPase-activating protein for Rap1 and Rap2. Segregate expression profiles from a rap1GAP gene product.
Kurachi H, Wada Y, Tsukamoto N, Maeda M, Kubota H, Hattori M, Iwai K, Minato N.
The Journal of Biological Chemistry 1997 Oct; 272(44):28081.
Application：IP, WB-Ce, Human, Mouse, 70z/3, HeLa, HL-60 cells.
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Bromodomain protein Brd4 binds to GTPase-activating SPA-1, modulating its activity and subcellular localization.
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