Sephs2 polyclonal antibody
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More Files
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Western Blot
Western blot using Sephs2 polyclonal antibody (Cat # PAB11323) shows detection of Sephs2 in NIH/3T3 cells over-expressing this protein.
No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with Sephs2 siRNA.
Endogenous Sephs2 can be detected in mouse kidney, liver and testes tissue lysates.
Partial cross-reactivity is seen against recombinant SPS1.
The primary antibody was used at a 1 : 1000 dilution.
Personal Communication, D. Hatfield, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant Sephs2.
Immunogen
Recombinant protein corresponding to full length mouse Sephs2.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody reacts with mouse SPS2 and shows partial cross-reactivity with SPS1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using Sephs2 polyclonal antibody (Cat # PAB11323) shows detection of Sephs2 in NIH/3T3 cells over-expressing this protein.
No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with Sephs2 siRNA.
Endogenous Sephs2 can be detected in mouse kidney, liver and testes tissue lysates.
Partial cross-reactivity is seen against recombinant SPS1.
The primary antibody was used at a 1 : 1000 dilution.
Personal Communication, D. Hatfield, NCI, Bethesda, MD.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Sephs2
Entrez GeneID
20768Protein Accession#
AAC53024;P97364Gene Name
Sephs2
Gene Alias
Sps2, Ysg3
Gene Description
selenophosphate synthetase 2
Gene Ontology
HyperlinkOther Designations
clone 1000|the SelD gene product
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Publication Reference
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Selenophosphate synthetase genes from lung adenocarcinoma cells: Sps1 for recycling L-selenocysteine and Sps2 for selenite assimilation.
Tamura T, Yamamoto S, Takahata M, Sakaguchi H, Tanaka H, Stadtman TC, Inagaki K.
PNAS 2004 Nov; 101(46):16162.
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Fetal mouse selenophosphate synthetase 2 (SPS2): biological activities of mutant forms in Escherichia coli.
Kim TS, Yu MH, Chung YW, Kim J, Choi EJ, Ahn K, Kim IY.
Molecules and Cells 1999 Aug; 9(4):422.
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Cloning and functional characterization of human selenophosphate synthetase, an essential component of selenoprotein synthesis.
Low SC, Harney JW, Berry MJ.
The Journal of Biological Chemistry 1995 Sep; 270(37):21659.
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Selenophosphate synthetase genes from lung adenocarcinoma cells: Sps1 for recycling L-selenocysteine and Sps2 for selenite assimilation.
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