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Last updated: 2017/7/23

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Ppard polyclonal antibody

  • Catalog # : PAB11322
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Ppard.
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus of mouse Ppard.
  • Host:
  • Rabbit
  • Reactivity:
  • Chimpanzee, Human, Mouse, Rabbit, Rat
  • Specificity:
  • This affinity purified antibody is directed against mouse PPAR delta protein. No reactivity is expected against other subtypes of PPAR.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:600000)
    Western Blot (1:500-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Immunoblotting of Ppard polyclonal antibody (Cat # PAB11322) is shown to detect a predominant band at ~ 43 kDa corresponding to Ppard present in mouse heart tissue lysates.
    Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown).
    Approximately 30 ug of lysate was loaded per lane for SDS-PAGE.
    Detection occurred using a 1 : 750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4°C followed reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green).
    IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • Ppard
  • Gene Alias:
  • NUC-1,NUC1,Nr1c2,PPAR-beta,PPAR-delta,PPAR[b],PPARdelta,Pparb,Pparb/d
  • Gene Description:
  • peroxisome proliferator activator receptor delta
  • Other Designations:
  • OTTMUSP00000027308,PPARdelta/beta,Peroxisome proliferator-activated receptor beta,mPPAR(delta)
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