Ppard polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Immunoblotting of Ppard polyclonal antibody (Cat # PAB11322) is shown to detect a predominant band at ~ 43 kDa corresponding to Ppard present in mouse heart tissue lysates.
Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown).
Approximately 30 ug of lysate was loaded per lane for SDS-PAGE.
Detection occurred using a 1 : 750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4°C followed reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green).
IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Ppard.
Immunogen
A synthetic peptide corresponding to N-terminus of mouse Ppard.
Host
Rabbit
Reactivity
Chimpanzee, Human, Mouse, Rabbit, Rat
Specificity
This affinity purified antibody is directed against mouse PPAR delta protein. No reactivity is expected against other subtypes of PPAR.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:600000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Immunoblotting of Ppard polyclonal antibody (Cat # PAB11322) is shown to detect a predominant band at ~ 43 kDa corresponding to Ppard present in mouse heart tissue lysates.
Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown).
Approximately 30 ug of lysate was loaded per lane for SDS-PAGE.
Detection occurred using a 1 : 750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4°C followed reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green).
IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Ppard
Entrez GeneID
19015Protein Accession#
P35396Gene Name
Ppard
Gene Alias
NUC-1, NUC1, Nr1c2, PPAR-beta, PPAR-delta, PPAR[b], PPARdelta, Pparb, Pparb/d
Gene Description
peroxisome proliferator activator receptor delta
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000027308|PPARdelta/beta|Peroxisome proliferator-activated receptor beta|mPPAR(delta)
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Publication Reference
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Medicine. PPARs as therapeutic targets: reverse cardiology?
Plutzky J.
Science 2003 Oct; 302(5644):406.
Application：WB-Tr, Human, Cancers, Mammalian cells.
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Transcriptional repression of atherogenic inflammation: modulation by PPARdelta.
Lee CH, Chawla A, Urbiztondo N, Liao D, Boisvert WA, Evans RM, Curtiss LK.
Science 2003 Oct; 302(5644):453.
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Progress in cardiovascular biology: PPAR for the course.
Lazar MA.
Nat Med 2001 Jan; 7(1):23.
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Peroxisome proliferator-activated receptors (PPARs): nuclear receptors at the crossroads between lipid metabolism and inflammation.
Chinetti G, Fruchart JC, Staels B.
Inflammation Research 2000 Oct; 49(10):497.
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Medicine. PPARs as therapeutic targets: reverse cardiology?
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