Ppara polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Immunoblotting of Ppara polyclonal antibody (Cat # PAB11321) is shown to detect a 52 kDa band corresponding to Ppara present in a NIH/3T3 whole cell lysate.
Approximately 20 ug of lysate was loaded per lane for SDS-PAGE.
Detection occurred after using a 1 : 500 (lane 1) or 1 : 1000 (lane 2) dilution of antibody followed by 1 : 2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Ppara.
Immunogen
A synthetic peptide corresponding to amino acids 1-18 of mouse Ppara.
Host
Rabbit
Reactivity
Bovine, Chimpanzee, Dog, Hamster, Human, Monkey, Mouse, Pig, Rat
Specificity
This affinity purified antibody is directed against mouse PPAR alpha protein. No reactivity is expected against other subtypes of PPAR.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000-1:32000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Immunoblotting of Ppara polyclonal antibody (Cat # PAB11321) is shown to detect a 52 kDa band corresponding to Ppara present in a NIH/3T3 whole cell lysate.
Approximately 20 ug of lysate was loaded per lane for SDS-PAGE.
Detection occurred after using a 1 : 500 (lane 1) or 1 : 1000 (lane 2) dilution of antibody followed by 1 : 2000 dilution of HRP Goat-a-Rabbit IgG for visualization.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Ppara
Entrez GeneID
19013Protein Accession#
P23204;NP_035274;NP_001001930Gene Name
Ppara
Gene Alias
4933429D07Rik, AW742785, Nr1c1, PPAR-alpha, PPARalpha, Ppar
Gene Description
peroxisome proliferator activated receptor alpha
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000027777|OTTMUSP00000027778|OTTMUSP00000027779
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Publication Reference
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Cardiac-specific ablation of the E3 ubiquitin ligase Mdm2 leads to oxidative stress, broad mitochondrial deficiency and early death.
Hauck L, Stanley-Hasnain S, Fung A, Grothe D, Rao V, Mak TW, Billia F.
PLoS One 2017 Dec; 12(12):e0189861.
Application：WB-Ti, Mouse, Mouse left ventricular tissue samples.
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Medicine. PPARs as therapeutic targets: reverse cardiology?
Plutzky J.
Science 2003 Oct; 302(5644):406.
Application：WB-Tr, Human, Cancers, Mammalian cells.
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Transcriptional repression of atherogenic inflammation: modulation by PPARdelta.
Lee CH, Chawla A, Urbiztondo N, Liao D, Boisvert WA, Evans RM, Curtiss LK.
Science 2003 Oct; 302(5644):453.
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Progress in cardiovascular biology: PPAR for the course.
Lazar MA.
Nat Med 2001 Jan; 7(1):23.
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Peroxisome proliferator-activated receptors (PPARs): nuclear receptors at the crossroads between lipid metabolism and inflammation.
Chinetti G, Fruchart JC, Staels B.
Inflammation Research 2000 Oct; 49(10):497.
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Cardiac-specific ablation of the E3 ubiquitin ligase Mdm2 leads to oxidative stress, broad mitochondrial deficiency and early death.
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