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Last updated: 2017/10/15
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Ppara polyclonal antibody

  • Catalog # : PAB11321
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Ppara.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 1-18 of mouse Ppara.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chimpanzee, Dog, Hamster, Human, Monkey, Mouse, Pig, Rat
  • Specificity:
  • This affinity purified antibody is directed against mouse PPAR alpha protein. No reactivity is expected against other subtypes of PPAR.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:8000-1:32000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblotting of Ppara polyclonal antibody (Cat # PAB11321) is shown to detect a 52 kDa band corresponding to Ppara present in a NIH/3T3 whole cell lysate.
    Approximately 20 ug of lysate was loaded per lane for SDS-PAGE.
    Detection occurred after using a 1 : 500 (lane 1) or 1 : 1000 (lane 2) dilution of antibody followed by 1 : 2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • P23204;NP_035274;NP_001001930
  • Gene Name:
  • Ppara
  • Gene Alias:
  • 4933429D07Rik,AW742785,Nr1c1,PPAR-alpha,PPARalpha,Ppar
  • Gene Description:
  • peroxisome proliferator activated receptor alpha
  • Other Designations:
  • OTTMUSP00000027777,OTTMUSP00000027778,OTTMUSP00000027779
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