Pogz polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Pogz.
Immunogen
A synthetic peptide corresponding to N-terminus of mouse Pogz.
Host
Rabbit
Reactivity
Baboons, Bovine, Chicken, Chimpanzee, Dog, Human, Macaque, Mouse, Opossum, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:100000-1:400000)
Western Blot (1:2000-1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using Pogz polyclonal antibody (Cat # PAB11320) shows detection of Pogz protein (arrowhead) in adult mouse thymus and spleen tissue lysate (Panel A).
The lower molecular weight bands may be cross reactive proteins.
Pre-incubation of antibody with the immunizing peptide blocks specific antibody reactivity (Panel B).
The primary antibody was used at 1 : 20,000.
Personal Communication, K.O. Gudmundsson and J. Keller, NCI, Frederick, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Pogz
Entrez GeneID
229584Protein Accession#
NP_766271;NM_172683;Q8BZH4Gene Name
Pogz
Gene Alias
9530006B08Rik, AU015913, AU044539
Gene Description
pogo transposable element with ZNF domain
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000021253
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