RFA2 (phospho S122) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blotting against RFA2 containing Saccharomyces sp. cell lysates. RFA2 (phospho S122) polyclonal antibody (Cat # PAB11311) at 1.0 ug/mL for 1 hr at RT followed by IRDye 800 Sh anti Rabbit IgG 1 : 5000 1 hr RT.
Western Blot (Cell lysate)
Western blotting against RFA2 containing Saccharomyces sp. cell lysates. RFA2 (phospho S122) polyclonal antibody (Cat # PAB11311) at 1.0 ug/mL for 1 hr at RT followed by IRDye 800 Sh anti Rabbit IgG 1 : 5000 1 hr RT.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of RFA2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S122 of Saccharomyces cerevisiae RFA2.
Host
Rabbit
Reactivity
Yeast
Specificity
This phospho specific polyclonal antibody is specific to phosphorylated pS122 of yeast RFA2. Reactivity with non-phosphorylated yeast RFA2 is minimal by ELISA.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000-1:20000 of phosphorylated form 1:300 of non-phosphorylated form)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blotting against RFA2 containing Saccharomyces sp. cell lysates. RFA2 (phospho S122) polyclonal antibody (Cat # PAB11311) at 1.0 ug/mL for 1 hr at RT followed by IRDye 800 Sh anti Rabbit IgG 1 : 5000 1 hr RT.Western Blot (Cell lysate)
Western blotting against RFA2 containing Saccharomyces sp. cell lysates. RFA2 (phospho S122) polyclonal antibody (Cat # PAB11311) at 1.0 ug/mL for 1 hr at RT followed by IRDye 800 Sh anti Rabbit IgG 1 : 5000 1 hr RT.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — RFA2
Entrez GeneID
855404Gene Name
RFA2
Gene Alias
BUF1, RPA2
Gene Description
Rfa2p
Gene Ontology
HyperlinkGene Summary
which is a highly conserved single-stranded DNA binding protein involved in DNA replication
Other Designations
Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination
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