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Last updated: 2017/8/20
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RFA2 (phospho S122) polyclonal antibody

  • Catalog # : PAB11311
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of RFA2.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S122 of Saccharomyces cerevisiae RFA2.
  • Host:
  • Rabbit
  • Reactivity:
  • Yeast
  • Specificity:
  • This phospho specific polyclonal antibody is specific to phosphorylated pS122 of yeast RFA2. Reactivity with non-phosphorylated yeast RFA2 is minimal by ELISA.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:8000-1:20000 of phosphorylated form 1:300 of non-phosphorylated form)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blotting against RFA2 containing Saccharomyces sp. cell lysates. RFA2 (phospho S122) polyclonal antibody (Cat # PAB11311) at 1.0 ug/mL for 1 hr at RT followed by IRDye 800 Sh anti Rabbit IgG 1 : 5000 1 hr RT.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blotting against RFA2 containing Saccharomyces sp. cell lysates. RFA2 (phospho S122) polyclonal antibody (Cat # PAB11311) at 1.0 ug/mL for 1 hr at RT followed by IRDye 800 Sh anti Rabbit IgG 1 : 5000 1 hr RT.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Gene Name:
  • RFA2
  • Gene Alias:
  • BUF1,RPA2
  • Gene Description:
  • Rfa2p
  • Other Designations:
  • Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination
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