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Last updated: 2016/12/4
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MER2 (phospho S30) polyclonal antibody

  • Catalog # : PAB11308
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of MER2.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S30 of Saccharomyces cerevisiae MER2.
  • Host:
  • Rabbit
  • Reactivity:
  • Yeast
  • Specificity:
  • Reactivity occurs against Saccharomyces cerevisiae Mer2 pS30 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated Saccharomyces cerevisiae Mer2 is minimal by ELISA and western blot.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:25000)
    Western Blot (1:1000-1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using MER2 (phospho S30) polyclonal antibody (Cat # PAB11308) shows detection of phosphorylated MER2 in whole cell extracts.
    Cells were either wild type (lane 1) or contained MER2 deletions (lane 2).
    Extracts were prepared from cells 4 hr after initiation of meiosis.
    Proteins were obtained using TCA precipitation.
    The primary antibody was used at a 1 : 7,500 dilution.
    Secondary antibody was used at 1:5,000 dilution.
    Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
  • Immunoprecipitation
  • Immunoprecipitation
  • Western blot using MER2 (phospho S30) polyclonal antibody (Cat # PAB11308) shows detection of phos-phorylated MER2, but not phosphatase treated or mutant cells.
    Lane 1 contains MER2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody.
    Cells were harvested 4 hrs after the initiation of meiosis and therefore contain mostly phosphorylated MER2.
    Lane 2 contains the same preparation after treatment with phosphatase.
    Lane 3 contains MER2-S30A protein as a phosphorylation control.
    This antibody appears to be specificfor phosphorylated MER2 at the S30 position with negligible cross reactivity against unphosphorylated protein.
    The primary antibody was used at a 1 : 5,000 dilution.
    Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • REC107
  • Gene Alias:
  • MER2
  • Gene Description:
  • Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation
  • Other Designations:
  • Rec107p
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