Rabbit polyclonal antibody raised against synthetic phosphopeptide of MER2.
Synthetic phosphopeptide corresponding to residues surrounding S30 of Saccharomyces cerevisiae MER2.
Reactivity occurs against Saccharomyces cerevisiae Mer2 pS30 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated Saccharomyces cerevisiae Mer2 is minimal by ELISA and western blot.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:5000-1:25000) Western Blot (1:1000-1:10000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using MER2 (phospho S30) polyclonal antibody (Cat # PAB11308) shows detection of phosphorylated MER2 in whole cell extracts. Cells were either wild type (lane 1) or contained MER2 deletions (lane 2). Extracts were prepared from cells 4 hr after initiation of meiosis. Proteins were obtained using TCA precipitation. The primary antibody was used at a 1 : 7,500 dilution. Secondary antibody was used at 1:5,000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
Western blot using MER2 (phospho S30) polyclonal antibody (Cat # PAB11308) shows detection of phos-phorylated MER2, but not phosphatase treated or mutant cells. Lane 1 contains MER2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 hrs after the initiation of meiosis and therefore contain mostly phosphorylated MER2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains MER2-S30A protein as a phosphorylation control. This antibody appears to be specificfor phosphorylated MER2 at the S30 position with negligible cross reactivity against unphosphorylated protein. The primary antibody was used at a 1 : 5,000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation