Western blot using Stat5a (phospho Y694) polyclonal antibody (Cat # PAB11307) shows detection of phosphorylated Stat5a (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1 ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5a in untreated cells (lane 1). The primary antibody at a 1 : 1,000 dilution, overnight at 4°C. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.
Immunohistochemistry of Stat5a (phospho Y694) polyclonal antibody (Cat # PAB11307) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of distal convoluted tubules and collecting ducts of human kidney at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.