STAT2 (phospho Y690) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using STAT2 (phospho Y690) polyclonal antibody (Cat # PAB11305) shows detection of STAT2 pY690 protein (arrowhead) in Jurkat cells without (left lane) and with (right lane) 1000 U/mL of IFN-alpha for 15 min at 37°C.
The primary antibody was used at 1 : 1,000.
Personal Communication, A.Gamero, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of STAT2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y690 of human STAT2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This affinity purified antibody is directed against human STAT2 pY690 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:85000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using STAT2 (phospho Y690) polyclonal antibody (Cat # PAB11305) shows detection of STAT2 pY690 protein (arrowhead) in Jurkat cells without (left lane) and with (right lane) 1000 U/mL of IFN-alpha for 15 min at 37°C.
The primary antibody was used at 1 : 1,000.
Personal Communication, A.Gamero, NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — STAT2
Entrez GeneID
6773Protein Accession#
NP_005410;P52630Gene Name
STAT2
Gene Alias
ISGF-3, MGC59816, P113, STAT113
Gene Description
signal transducer and activator of transcription 2, 113kDa
Omim ID
600556Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. [provided by RefSeq
Other Designations
interferon alpha induced transcriptional activator|signal transducer and activator of transcription 2
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Interactome
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Pathway
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Disease
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Publication Reference
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Functional subdomains of STAT2 required for preassociation with the alpha interferon receptor and for signaling.
Li X, Leung S, Kerr IM, Stark GR.
Molecular and Cellular Biology 1997 Apr; 17(4):2048.
Application：WB-Tr, Human, U6A cells.
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Functional subdomains of STAT2 required for preassociation with the alpha interferon receptor and for signaling.
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