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Last updated: 2017/12/10
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ROCK2 (phospho Y256) polyclonal antibody

  • Catalog # : PAB11303
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of ROCK2.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding Y256 of human ROCK2.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chicken, Dog, Frog, Fruit fly, Human, Mouse, Rat
  • Specificity:
  • This antibody is specific to human ROCK-2 protein phosphorylated at Y256.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000-1:10000)
    Western Blot (1:200-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using ROCK2 (phospho Y256) polyclonal antibody (Cat # PAB11303) shows detection of phosphorylated ROCK2 in transfected 293T cells.
    Lane 1 shows lysate expressing exogenous ROCK2 where the insert contains the transversion Y256A.
    Lane 2 shows the lysate expressing WT ROCK2.
    The primary antibody was used at a 1 : 1000 dilution.
    Personal Communication, A. Papageorge and X. Qian, NCI, Bethesda, MD.
  • Immunohistochemistry
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry
  • Gene Information
  • Entrez GeneID:
  • 9475
  • Protein Accession#:
  • BAA75636;O75116
  • Gene Name:
  • ROCK2
  • Gene Alias:
  • KIAA0619
  • Gene Description:
  • Rho-associated, coiled-coil containing protein kinase 2
  • Gene Summary:
  • The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000195104
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