RFX5 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
RFX5 polyclonal antibody (Cat # PAB11300) (amino acids 320-494) is shown to detect RFX5 present in Raji B cell nuclear extract lysates.
Detection occurs using a 1 : 2,500 dilution of antibody.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RFX5.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 320-494 of human RFX5.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
Gel Supershift assay (0.5-1.0 ul per assay)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
RFX5 polyclonal antibody (Cat # PAB11300) (amino acids 320-494) is shown to detect RFX5 present in Raji B cell nuclear extract lysates.
Detection occurs using a 1 : 2,500 dilution of antibody.Enzyme-linked Immunoabsorbent Assay
Gel Supershift Assay
-
Gene Info — RFX5
Entrez GeneID
5993Gene Name
RFX5
Gene Alias
-
Gene Description
regulatory factor X, 5 (influences HLA class II expression)
Gene Ontology
HyperlinkGene Summary
A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq
Other Designations
OTTHUMP00000082795|OTTHUMP00000196318|regulatory factor X, 5
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
A novel DNA-binding regulatory factor is mutated in primary MHC class II deficiency (bare lymphocyte syndrome).
Steimle V, Durand B, Barras E, Zufferey M, Hadam MR, Mach B, Reith W.
Genes & Development 1995 May; 9(9):1021.
-
A novel DNA-binding regulatory factor is mutated in primary MHC class II deficiency (bare lymphocyte syndrome).
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com