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Last updated: 2017/5/21
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NFYA polyclonal antibody

  • Catalog # : PAB11297
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of NFYA.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human NFYA.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol.
    Equivalent aliquots of chromatin from each sample containing ~50 ug of total protein were subjected to analysis by SDS-PAGE and immunoblot.
    Using NFYA polyclonal antibody (Cat # PAB11297) at a 1 : 1000 dilution.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4800
  • Gene Name:
  • NFYA
  • Gene Alias:
  • CBF-A,CBF-B,FLJ11236,HAP2,NF-YA
  • Gene Description:
  • nuclear transcription factor Y, alpha
  • Gene Summary:
  • The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq
  • Other Designations:
  • CAAT-box DNA binding protein subunit A,CCAAT-binding transcription factor subunit B,HAP2 CCAAT-binding protein,OTTHUMP00000016365,Transcription factor NF-Y, A subunit
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