Western blot using WNT1 polyclonal antibody (Cat # PAB11291) shows detection of endogenous WNT1 in human-derived MCF-7 cell lysate (lane 1) and mouse-derived 3T3 cell lysate (lane 2). The band at ~41 kDa, indicated by the arrowhead, corresponds to WNT1. After transfer, the membrane was blocked with 5% BLOTTO. Primary antibody was used at a 1 : 1,400 dilution in PBS containing 1% BLOTTO. The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with WNT1 (data not shown).
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. [provided by RefSeq
Wingless-type MMTV integration site family, member 1 (oncogene INT1)