Product Browser

Last updated: 2017/7/16
  • Related Product Showcase

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

VDAC1 polyclonal antibody

  • Catalog # : PAB11290
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of VDAC1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 185-197 of human VDAC1.
  • Host:
  • Rabbit
  • Reactivity:
  • Birds, Human
  • Specificity:
  • This affinity purified antibody is directed against human VDAC1/Porin1 protein. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms).
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:40000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using VDAC1 polyclonal antibody (Cat # PAB11290) detects a single band at ~32 KDa corresponding to VDAC1 (arrowhead) present in a rat heart tissue lysate.
    Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
    After blocking, the membrane was probed with the primary antibody diluted to 1 : 1,200 at 4°C overnight.
    Washes were followed by reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7416
  • Protein Accession#:
  • P21796(VDAC1);P45880(VDAC2)(isoform1);Q9Y277(VDAC3)(isoform1)
  • Gene Name:
  • VDAC1
  • Gene Alias:
  • MGC111064,PORIN,PORIN-31-HL
  • Gene Description:
  • voltage-dependent anion channel 1
  • Other Designations:
  • OTTHUMP00000165946,OTTHUMP00000165947
  • RSS
  • YouTube
  • Linkedin
  • Facebook