Western blot using VDAC1 polyclonal antibody (Cat # PAB11290) detects a single band at ~32 KDa corresponding to VDAC1 (arrowhead) present in a rat heart tissue lysate. Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1 : 1,200 at 4°C overnight. Washes were followed by reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.