UBE2J1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using UBE2J1 polyclonal antibody (Cat # PAB11288) shows detection of UBE2J1 in 293 cells over-expressing Myc-UBE2J1 (Lane 2).
Lane 1 contains lysate from mock-transfected 293 cells.
Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of UBE2J1.
Immunogen
A synthetic peptide corresponding to N-terminus of human UBE2J1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:1000-1:5000)
Western Blot (1:200-1:2000)
Immunoprecipitation (1-2 ug)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using UBE2J1 polyclonal antibody (Cat # PAB11288) shows detection of UBE2J1 in 293 cells over-expressing Myc-UBE2J1 (Lane 2).
Lane 1 contains lysate from mock-transfected 293 cells.
Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MD.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — UBE2J1
Entrez GeneID
51465Protein Accession#
NP_057105;Q9Y385Gene Name
UBE2J1
Gene Alias
CGI-76, HSPC153, HSPC205, HSU93243, MGC12555, NCUBE1, Ubc6p
Gene Description
ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast)
Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq
Other Designations
OTTHUMP00000016852|non-canonical ubquitin conjugating enzyme 1|ubiquitin-conjugating enzyme E2, J1
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Interactome
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Pathway
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Publication Reference
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A role for mammalian Ubc6 homologues in ER-associated protein degradation.
Lenk U, Yu H, Walter J, Gelman MS, Hartmann E, Kopito RR, Sommer T.
Journal of Cell Science 2002 Jul; 115(Pt 14):3007.
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Ubiquitin and its kin: how close are the family ties?
Jentsch S, Pyrowolakis G.
Trends in Cell Biology 2000 Aug; 10(8):335.
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Identification of a family of noncanonical ubiquitin-conjugating enzymes structurally related to yeast UBC6.
Lester D, Farquharson C, Russell G, Houston B.
Biochemical and Biophysical Research Communications 2000 Mar; 269(2):474.
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A role for mammalian Ubc6 homologues in ER-associated protein degradation.
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