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Last updated: 2017/1/15
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UBE2J1 polyclonal antibody

  • Catalog # : PAB11288
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of UBE2J1.
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus of human UBE2J1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:1000-1:5000)
    Western Blot (1:200-1:2000)
    Immunoprecipitation (1-2 ug)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using UBE2J1 polyclonal antibody (Cat # PAB11288) shows detection of UBE2J1 in 293 cells over-expressing Myc-UBE2J1 (Lane 2).
    Lane 1 contains lysate from mock-transfected 293 cells.
    Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MD.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_057105;Q9Y385
  • Gene Name:
  • UBE2J1
  • Gene Alias:
  • CGI-76,HSPC153,HSPC205,HSU93243,MGC12555,NCUBE1,Ubc6p
  • Gene Description:
  • ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast)
  • Gene Summary:
  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000016852,non-canonical ubquitin conjugating enzyme 1,ubiquitin-conjugating enzyme E2, J1
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