Western blot using UBE2J1 polyclonal antibody (Cat # PAB11288) shows detection of UBE2J1 in 293 cells over-expressing Myc-UBE2J1 (Lane 2). Lane 1 contains lysate from mock-transfected 293 cells. Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MD.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq