UBA1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using UBA1 polyclonal antibody (Cat # PAB11287) shows detection of a band at ~118 KDa corres-ponding to UBA1 (Lane 1 800 nm channel).
Approximately 35 ug of an A-431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 1,000.
Incubation was for 2 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™ 800 conjugated Gt-a-Rabbit IgG [H&L]MX10 for 45 min at room temperature.
Molecular weight markers are shown in lane 2 (700 nm channel).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of UBA1 polyclonal antibody (Cat # PAB11287) was used at a 10 ug/mL to detect UBA1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (isletand exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis,thymus, tonsil, and uterus (glandular, stroma).
In many cells a punctate nuclear staining was observed.
Other cells showedboth cytoplasmic and nuclear staining.
This image shows UBA1 staining of human lung tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communi-cation, TinaRoush, Life Span Biosciences, Seattle, WA.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant UBA1.
Immunogen
Recombinant GST fusion protein corresponding to full length human UBA1.
Host
Rabbit
Reactivity
Dog, Human, Mouse, Rabbit, Rat
Form
Lyophilized
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:1000-1:5000)
Immunohistochemistry (2-20 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C prior to restoration. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using UBA1 polyclonal antibody (Cat # PAB11287) shows detection of a band at ~118 KDa corres-ponding to UBA1 (Lane 1 800 nm channel).
Approximately 35 ug of an A-431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 1,000.
Incubation was for 2 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™ 800 conjugated Gt-a-Rabbit IgG [H&L]MX10 for 45 min at room temperature.
Molecular weight markers are shown in lane 2 (700 nm channel).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of UBA1 polyclonal antibody (Cat # PAB11287) was used at a 10 ug/mL to detect UBA1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (isletand exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis,thymus, tonsil, and uterus (glandular, stroma).
In many cells a punctate nuclear staining was observed.
Other cells showedboth cytoplasmic and nuclear staining.
This image shows UBA1 staining of human lung tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communi-cation, TinaRoush, Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — UBA1
Entrez GeneID
7317Protein Accession#
P22314;NP_003325Gene Name
UBA1
Gene Alias
A1S9, A1S9T, A1ST, AMCX1, GXP1, MGC4781, SMAX2, UBA1A, UBE1, UBE1X
Gene Description
ubiquitin-like modifier activating enzyme 1
Omim ID
314370Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq
Other Designations
A1S9T and BN75 temperature sensitivity complementing|OTTHUMP00000023197|OTTHUMP00000023198|UBA1, ubiquitin-activating enzyme E1 homolog A|ubiquitin-activating enzyme E1
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Interactome
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Pathway
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Disease
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Publication Reference
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Large-scale characterization of HeLa cell nuclear phosphoproteins.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villen J, Li J, Cohn MA, Cantley LC, Gygi SP.
PNAS 2004 Aug; 101(33):12130.
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Screening of Hsp105alpha-binding proteins using yeast and bacterial two-hybrid systems.
Saito Y, Doi K, Yamagishi N, Ishihara K, Hatayama T.
Biochemical and Biophysical Research Communications 2004 Feb; 314(2):396.
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A non-proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter.
Bres V, Kiernan RE, Linares LK, Chable-Bessia C, Plechakova O, Treand C, Emiliani S, Peloponese JM, Jeang KT, Coux O, Scheffner M, Benkirane M.
Nature Cell Biology 2003 Aug; 5(8):754.
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Large-scale characterization of HeLa cell nuclear phosphoproteins.
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