Western blot using UBA1 polyclonal antibody (Cat # PAB11287) shows detection of a band at ~118 KDa corres-ponding to UBA1 (Lane 1 800 nm channel). Approximately 35 ug of an A-431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 1,000. Incubation was for 2 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™ 800 conjugated Gt-a-Rabbit IgG [H&L]MX10 for 45 min at room temperature. Molecular weight markers are shown in lane 2 (700 nm channel). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.
Immunohistochemistry of UBA1 polyclonal antibody (Cat # PAB11287) was used at a 10 ug/mL to detect UBA1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (isletand exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis,thymus, tonsil, and uterus (glandular, stroma). In many cells a punctate nuclear staining was observed. Other cells showedboth cytoplasmic and nuclear staining. This image shows UBA1 staining of human lung tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communi-cation, TinaRoush, Life Span Biosciences, Seattle, WA.
The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq
A1S9T and BN75 temperature sensitivity complementing,OTTHUMP00000023197,OTTHUMP00000023198,UBA1, ubiquitin-activating enzyme E1 homolog A,ubiquitin-activating enzyme E1