UBE2N polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of UBE2N.
Immunogen
A synthetic peptide corresponding to amino acids 40-51 of human UBE2N.
Host
Goat
Reactivity
Bovine, Chicken, Chimpanzee, Clawed frog, Dog, Frog, Human, Macaque, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:25000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using UBE2N polyclonal antibody (Cat # PAB11286) shows detection of UBE2N protein inhuman small intestine lysate (Lane 1), but not in mouse thymus lysate (Lane 2).
The heavily stained band in lane 1 (arrowhead) indicates this particular gel was overloaded with protein.
The identity of minor reactive bands is unknown, but could represent E2 complexes.
Each lane contains approximately 20 ug of lysate.
Primary antibody was used at a 1 : 500 dilution.
The membrane was washed and reacted with a 1 : 10,000 dilution of Alexa Fluor™ 680 conjugated Rb-a-Goat IgG.Enzyme-linked Immunoabsorbent Assay
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Gene Info — UBE2N
Entrez GeneID
7334Protein Accession#
NP_003339;P61088Gene Name
UBE2N
Gene Alias
MGC131857, MGC8489, UBC13, UbcH-ben
Gene Description
ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
Omim ID
603679Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq
Other Designations
bendless-like ubiquitin conjugating enzyme|ubiquitin carrier protein N|ubiquitin-conjugating enzyme E2N|ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13)|ubiquitin-protein ligase N|yeast UBC13 homolog
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Chaperoned ubiquitylation--crystal structures of the CHIP U box E3 ubiquitin ligase and a CHIP-Ubc13-Uev1a complex.
Zhang M, Windheim M, Roe SM, Peggie M, Cohen P, Prodromou C, Pearl LH.
Molecular Cell 2005 Nov; 20(4):525.
Application:WB-Tr, Human, 293 cells.
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ISG15 modification of ubiquitin E2 Ubc13 disrupts its ability to form thioester bond with ubiquitin.
Zou W, Papov V, Malakhova O, Kim KI, Dao C, Li J, Zhang DE.
Biochemical and Biophysical Research Communications 2005 Oct; 336(1):61.
Application:WB-Tr, Human, HEK 293T cells.
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A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex.
Pastushok L, Moraes TF, Ellison MJ, Xiao W.
The Journal of Biological Chemistry 2005 May; 280(18):17891.
Application:WB, Saccharomyces cerevisiae, Yeast cells.
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Chaperoned ubiquitylation--crystal structures of the CHIP U box E3 ubiquitin ligase and a CHIP-Ubc13-Uev1a complex.
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