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Last updated: 2016/10/23
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UBE2N polyclonal antibody

  • Catalog # : PAB11286
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of UBE2N.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 40-51 of human UBE2N.
  • Host:
  • Goat
  • Reactivity:
  • Bovine, Chicken, Chimpanzee, Clawed frog, Dog, Frog, Human, Macaque, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:25000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using UBE2N polyclonal antibody (Cat # PAB11286) shows detection of UBE2N protein inhuman small intestine lysate (Lane 1), but not in mouse thymus lysate (Lane 2).
    The heavily stained band in lane 1 (arrowhead) indicates this particular gel was overloaded with protein.
    The identity of minor reactive bands is unknown, but could represent E2 complexes.
    Each lane contains approximately 20 ug of lysate.
    Primary antibody was used at a 1 : 500 dilution.
    The membrane was washed and reacted with a 1 : 10,000 dilution of Alexa Fluor™ 680 conjugated Rb-a-Goat IgG.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 7334
  • Protein Accession#:
  • NP_003339;P61088
  • Gene Name:
  • UBE2N
  • Gene Alias:
  • MGC131857,MGC8489,UBC13,UbcH-ben
  • Gene Description:
  • ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
  • Gene Summary:
  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq
  • Other Designations:
  • bendless-like ubiquitin conjugating enzyme,ubiquitin carrier protein N,ubiquitin-conjugating enzyme E2N,ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13),ubiquitin-protein ligase N,yeast UBC13 homolog
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