Products

Western Blot (Cell lysate)

Western blot using TAF1 polyclonal antibody (Cat # PAB11285) to detect TAF1 in HeLa nuclear extract (arrowhead).
The membrane was probed with the primary antibody at dilutions of 1 : 100 (lane 1), 1 : 250 (lane 2), 1 : 500 (Lane 3) and 1 : 1,000 (Lane 4).
The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1.
Personal Communication, Anne Gegonne, CCR-NCI, Bethesda, MD.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

6872

Protein Accession#

P21675

Gene Name

TAF1

Gene Alias

BA2R, CCG1, CCGS, DYT3, KAT4, N-TAF1, NSCL2, OF, P250, TAF2A, TAFII250

Gene Description

TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa

Omim ID

313650 314250

Gene Ontology

Hyperlink

Gene Summary

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. This gene is part of a complex transcriptional unit (TAF1/DYT3), wherein some products share exons with TAF1 as well as additional exons downstream.[provided by RefSeq

Other Designations

OTTHUMP00000024334|TBP-associated factor 1|cell cycle, G1 phase defect|complementation of cell cycle block, G1-to-S|transcription factor TFIID p250 polypeptide