Western blot using TMBIM1 polyclonal antibody (Cat # PAB11278) shows detection of exogenous TMBIM1 in lysates from HeLa cells transfected with pcDNA3-TMBIM1 (Lane 1). No staining is observed in lysates from mock transformed HeLa cells (Lane 2). To date this antibody has shown the ability to recognize overexpressed TMBIM1, but not endogenous protein. The primary antibody at a 1:1,000 dilution. Personal Communication from Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.