TMBIM1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using TMBIM1 polyclonal antibody (Cat # PAB11278) shows detection of exogenous TMBIM1 in lysates from HeLa cells transfected with pcDNA3-TMBIM1 (Lane 1).
No staining is observed in lysates from mock transformed HeLa cells (Lane 2).
To date this antibody has shown the ability to recognize overexpressed TMBIM1, but not endogenous protein.
The primary antibody at a 1:1,000 dilution.
Personal Communication from Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant TMBIM1.
Immunogen
Recombinant protein corresponding to N-terminus of human TMBIM1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using TMBIM1 polyclonal antibody (Cat # PAB11278) shows detection of exogenous TMBIM1 in lysates from HeLa cells transfected with pcDNA3-TMBIM1 (Lane 1).
No staining is observed in lysates from mock transformed HeLa cells (Lane 2).
To date this antibody has shown the ability to recognize overexpressed TMBIM1, but not endogenous protein.
The primary antibody at a 1:1,000 dilution.
Personal Communication from Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TMBIM1
Entrez GeneID
64114Protein Accession#
NP_071435;Q969X1Gene Name
TMBIM1
Gene Alias
MST100, MSTP100, PP1201, RECS1
Gene Description
transmembrane BAX inhibitor motif containing 1
Omim ID
610364Gene Ontology
HyperlinkOther Designations
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