THRB polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot using THRB polyclonal antibody (Cat # PAB11277) shows detection of in vitro translated THRB (lane 2) and human thyroid stable cell line expressing THTRB (lane 3).
No staining is evident in non transfected human thyroid cells (lane 1), over expressed THRA (lane 4) or HeLa stable cell line expressing THRA (lane 5).
The band at ~62 kDa, indicated by the arrowheads, corresponds to THRB.
Personal communication, H. Ying, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of THRB.
Immunogen
A synthetic peptide corresponding to N-terminus of human THRB.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:200000-1:220000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using THRB polyclonal antibody (Cat # PAB11277) shows detection of in vitro translated THRB (lane 2) and human thyroid stable cell line expressing THTRB (lane 3).
No staining is evident in non transfected human thyroid cells (lane 1), over expressed THRA (lane 4) or HeLa stable cell line expressing THRA (lane 5).
The band at ~62 kDa, indicated by the arrowheads, corresponds to THRB.
Personal communication, H. Ying, NCI, Bethesda, MD.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — THRB
Entrez GeneID
7068Protein Accession#
NP_000452.2;P10828Gene Name
THRB
Gene Alias
ERBA-BETA, ERBA2, GRTH, MGC126109, MGC126110, NR1A2, PRTH, THR1, THRB1, THRB2
Gene Description
thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000161044|avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2|oncogene ERBA2|thyroid hormone receptor beta 1|thyroid hormone receptor beta isoform|thyroid hormone receptor, beta
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Interactome
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Pathway
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Publication Reference
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Bisphenol A influences oestrogen- and thyroid hormone-regulated thyroid hormone receptor expression in rat cerebellar cell culture.
Somogyi V, Horváth TL, Tóth I, Bartha T, Frenyó LV, Kiss DS, Jócsák G, Kerti A, Naftolin F, Zsarnovszky A.
Acta veterinaria Hungarica 2016 Dec; 64(4):497.
Application：WB-Ce, Rat, Rat primary cerebellar cell cultures.
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Structural rearrangements in the thyroid hormone receptor hinge domain and their putative role in the receptor function.
Nascimento AS, Dias SM, Nunes FM, Aparicio R, Ambrosio AL, Bleicher L, Figueira AC, Santos MA, de Oliveira Neto M, Fischer H, Togashi M, Craievich AF, Garratt RC, Baxter JD, Webb P, Polikarpov I.
Journal of Molecular Biology 2006 Jul; 360(3):586.
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Pituitary resistance to thyroid hormone syndrome is associated with T3 receptor mutants that selectively impair beta2 isoform function.
Wan W, Farboud B, Privalsky ML.
Molecular Endocrinology (Baltimore, Md.) 2005 Jun; 19(6):1529.
Application：WB-Tr, Monkey, CV-1 cells.
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Physiological and molecular basis of thyroid hormone action.
Yen PM.
Physiological Reviews 2001 Jul; 81(3):1097.
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Bisphenol A influences oestrogen- and thyroid hormone-regulated thyroid hormone receptor expression in rat cerebellar cell culture.
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