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Last updated: 2017/1/22
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THRA polyclonal antibody

  • Catalog # : PAB11276
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of THRA.
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus of human THRA.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:650000)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using THRA polyclonal antibody (Cat # PAB11276) shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3).
    No staining is evident in lysates from mock-transfected 293 cells (lane 2).
    Endogenous THRA is not detected in mouse brain tissue lysate (lane 4).
    Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus.
    The band at ~55 kDa, indicated by the arrowhead, corresponds to THRA.
    Personal communication, S. Cheng and H. Ying, NCI, Bethesda, MD.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7067
  • Protein Accession#:
  • NP_955366;P10827-2
  • Gene Name:
  • THRA
  • Gene Alias:
  • AR7,EAR7,ERB-T-1,ERBA,ERBA1,MGC000261,MGC43240,NR1A1,THRA1,THRA2,c-ERBA-1
  • Gene Description:
  • thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)
  • Gene Summary:
  • The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
  • Other Designations:
  • ERBA-related 7,OTTHUMP00000164470,avian erythroblastic leukemia viral (v-erb-a) oncogene homolog,thyroid hormone receptor alpha,thyroid hormone receptor, alpha,triiodothyronine receptor
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