THRA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of THRA.
Immunogen
A synthetic peptide corresponding to N-terminus of human THRA.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:650000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using THRA polyclonal antibody (Cat # PAB11276) shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3).
No staining is evident in lysates from mock-transfected 293 cells (lane 2).
Endogenous THRA is not detected in mouse brain tissue lysate (lane 4).
Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus.
The band at ~55 kDa, indicated by the arrowhead, corresponds to THRA.
Personal communication, S. Cheng and H. Ying, NCI, Bethesda, MD.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — THRA
Entrez GeneID
7067Protein Accession#
NP_955366;P10827-2Gene Name
THRA
Gene Alias
AR7, EAR7, ERB-T-1, ERBA, ERBA1, MGC000261, MGC43240, NR1A1, THRA1, THRA2, c-ERBA-1
Gene Description
thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)
Omim ID
190120Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
ERBA-related 7|OTTHUMP00000164470|avian erythroblastic leukemia viral (v-erb-a) oncogene homolog|thyroid hormone receptor alpha|thyroid hormone receptor, alpha|triiodothyronine receptor
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Interactome
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Pathway
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Disease
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Publication Reference
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CREG1 stimulates brown adipocyte formation and ameliorates diet-induced obesity in mice.
Hashimoto M, Kusudo T, Takeuchi T, Kataoka N, Mukai T, Yamashita H.
FASEB Journal 2019 Jul; 33(7):8069.
Application:WB, Mouse, C3H10T1/2 cells.
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Bisphenol A influences oestrogen- and thyroid hormone-regulated thyroid hormone receptor expression in rat cerebellar cell culture.
Somogyi V, Horváth TL, Tóth I, Bartha T, Frenyó LV, Kiss DS, Jócsák G, Kerti A, Naftolin F, Zsarnovszky A.
Acta Veterinaria Hungarica 2016 Dec; 64(4):497.
Application:WB-Ce, Rat, Rat primary cerebellar cell cultures.
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Impaired adipogenesis caused by a mutated thyroid hormone alpha1 receptor.
Ying H, Araki O, Furuya F, Kato Y, Cheng SY.
Molecular and Cellular Biology 2007 Mar; 27(6):2359.
Application:WB, Mouse, 3T3-L1 cells.
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Structural rearrangements in the thyroid hormone receptor hinge domain and their putative role in the receptor function.
Nascimento AS, Dias SM, Nunes FM, Aparicio R, Ambrosio AL, Bleicher L, Figueira AC, Santos MA, de Oliveira Neto M, Fischer H, Togashi M, Craievich AF, Garratt RC, Baxter JD, Webb P, Polikarpov I.
Journal of Molecular Biology 2006 Jul; 360(3):586.
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Pituitary resistance to thyroid hormone syndrome is associated with T3 receptor mutants that selectively impair beta2 isoform function.
Wan W, Farboud B, Privalsky ML.
Molecular Endocrinology (Baltimore, Md.) 2005 Jun; 19(6):1529.
Application:WB-Tr, Monkey, CV-1 cells.
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CREG1 stimulates brown adipocyte formation and ameliorates diet-induced obesity in mice.
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