TYMS polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against recombinant TYMS.
Immunogen
Recombinant protein corresponding to human TYMS.
Host
Rabbit
Reactivity
Bacteria, Human, Monkey, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:1000)
ELISA (1:100000)
Immunohistochemistry (1:100)
Immunoprecipitation (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
TYMS polyclonal antibody (Cat # PAB11275) is shown to detect TYMS present in HeLa cell extract.
Each lane is estimated to contain 4 ug of protein.
Lanes 1, 2 and 3 represent 1:500, 1:1,000 and 1:2,000 fold dilutions of the antibody.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — TYMS
Entrez GeneID
7298Gene Name
TYMS
Gene Alias
HsT422, MGC88736, TMS, TS, TSase
Gene Description
thymidylate synthetase
Omim ID
188350Gene Ontology
HyperlinkGene Summary
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq
Other Designations
Thymidylate synthase
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Characterization of a polyclonal antibody to human thymidylate synthase suitable for the study of colorectal cancer specimens.
Haqqani AS, Cowling RT, Maroun JA, Birnboim HC.
The Journal of Histochemistry and Cytochemistry 1999 Dec; 47(12):1563.
Application:ELISA, ICC, IF, IHC-P, IP, WB-Ce, WB-Tr, Human, Monkey, Rat, COS-1, HEK 293T, HeLa, HL-60 cells Human colorectal cancer specimens, Neutrophils, OSE cells, Skin fibroblasts.
-
Characterization of a polyclonal antibody to human thymidylate synthase suitable for the study of colorectal cancer specimens.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com