SAE1 polyclonal antibody
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More Files
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Western Blot
Coomassie-stained SDS-PAGE of GST-SAE1 recombinant protein (Panel A) and western blotting (Panel B) of HeLa WC lysate (lane1) and purified recombinant GST-SAE1 (Lane 2) are presented to show specificity of SAE1 polyclonal antibody (Cat # PAB11270).
The ~60 KDa band present in ~ 35 ug lysate (green, 800 nm channel) is indicated by the arrowhead.
Lane 2 contains 50 ng of purified recombinant GST-SAE1 and lane 3 contains 300 ng of purified GST.Proteins were separated on a 4-20% Tris-Glycine gel by SDS-PAGE andtransferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 2,000.
Incubation was overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.
Molecular weight markers are shown for both the coomassie-stained gel and the western blot (Lane M, red, 700 nm channel).
IRDye™ 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is at rademark of LI-COR, Inc.
SDS-PAGE image courtesy of Proteome Resources, Englewood, CO, http://www.proteomeresources.com.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant SAE1.
Immunogen
Recombinant GST fusion protein corresponding to full length human SAE1.
Host
Rabbit
Reactivity
Bovine, Chimpanzee, Dog, Human, Mouse, Rat
Specificity
This purified antibody is directed against human SUMO Activating Enzyme E1 protein.
Form
Lyophilized
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot after reconstitution to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Coomassie-stained SDS-PAGE of GST-SAE1 recombinant protein (Panel A) and western blotting (Panel B) of HeLa WC lysate (lane1) and purified recombinant GST-SAE1 (Lane 2) are presented to show specificity of SAE1 polyclonal antibody (Cat # PAB11270).
The ~60 KDa band present in ~ 35 ug lysate (green, 800 nm channel) is indicated by the arrowhead.
Lane 2 contains 50 ng of purified recombinant GST-SAE1 and lane 3 contains 300 ng of purified GST.Proteins were separated on a 4-20% Tris-Glycine gel by SDS-PAGE andtransferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 2,000.
Incubation was overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.
Molecular weight markers are shown for both the coomassie-stained gel and the western blot (Lane M, red, 700 nm channel).
IRDye™ 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is at rademark of LI-COR, Inc.
SDS-PAGE image courtesy of Proteome Resources, Englewood, CO, http://www.proteomeresources.com.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — SAE1
Entrez GeneID
10055Protein Accession#
Q9UBE0Gene Name
SAE1
Gene Alias
AOS1, FLJ3091, HSPC140, SUA1
Gene Description
SUMO1 activating enzyme subunit 1
Gene Ontology
HyperlinkOther Designations
SUMO-1 activating enzyme E1 N subunit|SUMO-1 activating enzyme subunit 1|activator of SUMO1|sentrin/SUMO-activating protein AOS1|ubiquitin-like protein SUMO-1 activating enzyme
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Interactome
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Pathway
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Publication Reference
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Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1.
Lois LM, Lima CD.
The EMBO Journal 2005 Feb; 24(3):439.
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Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
Desterro JM, Rodriguez MS, Kemp GD, Hay RT.
The Journal of Biological Chemistry 1999 Apr; 274(15):10618.
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In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2.
Okuma T, Honda R, Ichikawa G, Tsumagari N, Yasuda H.
Biochemical and Biophysical Research Communications 1999 Jan; 254(3):693.
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Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1.
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