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Last updated: 2017/4/30
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SAE1 polyclonal antibody

  • Catalog # : PAB11270
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant SAE1.
  • Immunogen:
  • Recombinant GST fusion protein corresponding to full length human SAE1.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chimpanzee, Dog, Human, Mouse, Rat
  • Specificity:
  • This purified antibody is directed against human SUMO Activating Enzyme E1 protein.
  • Form:
  • Lyophilized
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot after reconstitution to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Coomassie-stained SDS-PAGE of GST-SAE1 recombinant protein (Panel A) and western blotting (Panel B) of HeLa WC lysate (lane1) and purified recombinant GST-SAE1 (Lane 2) are presented to show specificity of SAE1 polyclonal antibody (Cat # PAB11270).
    The ~60 KDa band present in ~ 35 ug lysate (green, 800 nm channel) is indicated by the arrowhead.
    Lane 2 contains 50 ng of purified recombinant GST-SAE1 and lane 3 contains 300 ng of purified GST.Proteins were separated on a 4-20% Tris-Glycine gel by SDS-PAGE andtransferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 2,000.
    Incubation was overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.
    Molecular weight markers are shown for both the coomassie-stained gel and the western blot (Lane M, red, 700 nm channel).
    IRDye™ 800 fluorescence image was capture
  • Immunofluorescence
  • ELISA
  • Application Image
  • Immunofluorescence
  • ELISA
  • Gene Information
  • Gene Name:
  • SAE1
  • Gene Alias:
  • AOS1,FLJ3091,HSPC140,SUA1
  • Gene Description:
  • SUMO1 activating enzyme subunit 1
  • Other Designations:
  • SUMO-1 activating enzyme E1 N subunit,SUMO-1 activating enzyme subunit 1,activator of SUMO1,sentrin/SUMO-activating protein AOS1,ubiquitin-like protein SUMO-1 activating enzyme
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