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SPANXC polyclonal antibody

  • Catalog # : PAB11268
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant SPANXC.
  • Immunogen:
  • Recombinant protein corresponding to full length human SPANXC.
  • Host:
  • Rabbit
  • Reactivity:
  • Chimpanzee, Gorilla, Human
  • Specificity:
  • This Protein A purified antibody is directed against human SPANX-C protein.
  • Form:
  • Lyophilized
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:1000-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot after reconstitution to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using SPANXC polyclonal antibody (Cat # PAB11268) shows detection of a band at ~17 KDa corresponding to SPANXC present in a nuclear extract from VWM105 cells (left panel, arrowhead).
    VWM105 cells are derived from a human melanoma and are positive for SPANX proteins.
    Lane 2 shows reactivity with a purified recombinant SPANXC fusion protein.
    The right panel shows similar reactivity with purified recombinant SPANXB, SPANXC and SPANXN proteins.
    Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1 : 1,000.
    IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX was used (left).
    IRDye is a trademark of LI-COR, Inc.
    Size estimation was made by comparison to prestained MW markers as indicated.
    Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of SPANXC polyclonal antibody (Cat # PAB11268) was used at 2.5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
    This image shows moderate positive staining of human sperm and spermatids at 60X.
    Tissue was formalin-fixed and paraffin embedded.
    The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
    Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
  • ELISA
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • CAD29839;CAB75344;CAI41662;Q9NY87
  • Gene Name:
  • SPANXC
  • Gene Alias:
  • C,CTp11,SPANX-C
  • Gene Description:
  • SPANX family, member C
  • Gene Summary:
  • Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family, which is located in a gene cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene encodes a protein that localizes to the nucleus and is expressed in highly metastatic cell lines, making the protein a potential diagnostic and prognostic marker. The protein belongs to a family of cancer/testis antigens and represents a potential target for cancer immunotherapy. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000024173,SPAN-Xc protein,cancer-testis-associated protein CTp11,cancer/testis-associated protein of 11 kD,sperm protein associated with the nucleus, X chromosome, family member C
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