Western blot using SPANXC polyclonal antibody (Cat # PAB11267) shows detection of a band at ~17 KDacorresponding to SPANXC present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells arederived from a human melanoma and arepositive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANXC fusion protein. The right panel shows similar reactivity with purified recombinant SPANXB, SPANXC and SPANXN proteins. Proteins were separatedby SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1 : 1,000. IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers asindicated. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.
Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family, which is located in a gene cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene encodes a protein that localizes to the nucleus and is expressed in highly metastatic cell lines, making the protein a potential diagnostic and prognostic marker. The protein belongs to a family of cancer/testis antigens and represents a potential target for cancer immunotherapy. [provided by RefSeq
OTTHUMP00000024173,SPAN-Xc protein,cancer-testis-associated protein CTp11,cancer/testis-associated protein of 11 kD,sperm protein associated with the nucleus, X chromosome, family member C