RBL2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using RBL2 polyclonal antibody (Cat # PAB11266) shows detection of endogenous RBL2 protein in whole LNCaP cell extracts.
The band at ~130 KDa (arrowhead).
The primary antibody was diluted 1 : 500.
Personal communication, Ang Sun, Sbarro Institute for Cancer Research and Molecular Medicine, Temple University, Philadelphia, PA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RBL2.
Immunogen
A synthetic peptide corresponding to the Spa310 domain of human RBL2.
Host
Rabbit
Reactivity
Chimpanzee, Human, Orangutan
Specificity
This antiserum is directed against Spa310 and reacts with the Spa3120 domain of pRb2/p130 from human tissues.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:75000-1:100000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using RBL2 polyclonal antibody (Cat # PAB11266) shows detection of endogenous RBL2 protein in whole LNCaP cell extracts.
The band at ~130 KDa (arrowhead).
The primary antibody was diluted 1 : 500.
Personal communication, Ang Sun, Sbarro Institute for Cancer Research and Molecular Medicine, Temple University, Philadelphia, PA.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — RBL2
Entrez GeneID
5934Protein Accession#
CAA52671;Q08999Gene Name
RBL2
Gene Alias
FLJ26459, P130, Rb2
Gene Description
retinoblastoma-like 2 (p130)
Omim ID
180203Gene Ontology
HyperlinkOther Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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From G0 to S phase: a view of the roles played by the retinoblastoma (Rb) family members in the Rb-E2F pathway.
Sun A, Bagella L, Tutton S, Romano G, Giordano A.
Journal of Cellular Biochemistry 2007 Dec; 102(6):1400.
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A small molecule based on the pRb2/p130 spacer domain leads to inhibition of cdk2 activity, cell cycle arrest and tumor growth reduction in vivo.
Bagella L, Sun A, Tonini T, Abbadessa G, Cottone G, Paggi MG, De Luca A, Claudio PP, Giordano A.
Oncogene 2007 Mar; 26(13):1829.
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Frequent loss of pRb2/p130 in human ovarian carcinoma.
D'Andrilli G, Masciullo V, Bagella L, Tonini T, Minimo C, Zannoni GF, Giuntoli RL 2nd, Carlson JA Jr, Soprano DR, Soprano KJ, Scambia G, Giordano A.
Clinical Cancer Research 2004 May; 10(9):3098.
Application：IHC-P, WB-Ti, Human, A2780, CAOV3 cells, Human ovarian carcinoma.
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From G0 to S phase: a view of the roles played by the retinoblastoma (Rb) family members in the Rb-E2F pathway.
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