SMAD2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SMAD2.
Immunogen
A synthetic peptide corresponding to internal region of human SMAD2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is Smad2 specific, and reactivity to other Smad proteins (specifically Smad1, Smad3, Smad4, and Smad7) is not detected in over-expressed cell lysates.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:100000)
Western Blot (1:1000-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using SMAD2 polyclonal antibody (Cat # PAB11262) to detect over-expressed SMAD2 in COS cells (arrow).
Lane 1 shows mock infection of COS cells with lentiviral vector alone.
Lane 2 shows detection of SMAD2 in lysates of COS transfected with SMAD2.
Lane 3 contains lysates of MDA-MB231 cells treated with vehicle.
Lane 4 contains lysate of MDA-MB231 cells treated with TGF beta.
The expected MW for SMAD2 is 52 kDa.
The membrane was probed with the primary antibody at a 1:2500 dilution.
Personal Communication Kathleen Flanders, CCR-NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SMAD2
Entrez GeneID
4087Protein Accession#
NP_005892;Q15796Gene Name
SMAD2
Gene Alias
JV18, JV18-1, MADH2, MADR2, MGC22139, MGC34440, hMAD-2, hSMAD2
Gene Description
SMAD family member 2
Omim ID
601366Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
MAD, mothers against decapentaplegic homolog 2|Mad protein homolog|Mad, mothers against decapentaplegic homolog 2|Mad-related protein 2|SMAD, mothers against DPP homolog 2|Sma- and Mad-related protein 2|mother against DPP homolog 2
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Interactome
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Pathway
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Disease
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Publication Reference
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Effect of the different phosphorylated Smad2 protein localizations on the invasive breast carcinoma phenotype.
Liapis G, Mylona E, Alexandrou P, Giannopoulou I, Nikolaou I, Markaki S, Keramopoulos A, Nakopoulou L.
APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica 2007 Feb; 115(2):104.
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High TGFbeta-Smad activity confers poor prognosis in glioma patients and promotes cell proliferation depending on the methylation of the PDGF-B gene.
Bruna A, Darken RS, Rojo F, Ocana A, Penuelas S, Arias A, Paris R, Tortosa A, Mora J, Baselga J, Seoane J.
Cancer Cell 2007 Feb; 11(2):147.
Application:IHC, Human, Human glioma sections.
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Leptin augments myofibroblastic conversion and fibrogenic activity of human peritoneal mesothelial cells: a functional implication for peritoneal fibrosis.
Yang AH, Huang SW, Chen JY, Lin JK, Chen CY.
Nephrology, Dialysis, Transplantation 2007 Mar; 22(3):756.
Application:WB-Ce, Human, Human peritoneal mesothelial cells.
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Effect of the different phosphorylated Smad2 protein localizations on the invasive breast carcinoma phenotype.
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