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Last updated: 2017/6/18
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SLIT3 polyclonal antibody

  • Catalog # : PAB11260
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SLIT3.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 1164-1177 of human SLIT3.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chicken, Chimpanzee, Dog, Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000-1:10000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using SLIT3 polyclonal antibody (Cat # PAB11260) shows detection ofa predominant band at ~145 KDa corresponding to SLIT3 (arrowhead) in a bovine thyroid tissue lysate using the 800 nm channel (green).
    Approximately 35 ug of lysate was separated on a 4-8% Tricine gel by SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1 : 800.
    Incubation was for 2 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™ 800 conjugated Gt-a-RabbitIgG [H&L] MXHu for 45 min atroom temperature.
    Molecular weight markers were used for size comparison using the 700 nm channel (not shown).
    IRDye™ 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye isa trademark of LI-COR, Inc.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6586
  • Protein Accession#:
  • O75094;NP_003053
  • Gene Name:
  • SLIT3
  • Gene Alias:
  • FLJ10764,MEGF5,SLIL2,SLIT1,Slit-3,slit2
  • Gene Description:
  • slit homolog 3 (Drosophila)
  • Other Designations:
  • slit homolog 3
  • Gene Pathway
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