SLIT2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SLIT2.
Immunogen
A synthetic peptide corresponding to amino acids 484-500 of human SLIT2.
Host
Rabbit
Reactivity
Bovine, Chicken, Clawed frog, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:1500-1:6000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using SLIT2 polyclonal antibody (Cat # PAB11259) shows detection of a band at ~165 KDa (Lane 1) corresponding to SLIT2 present in a chicken spinal cord whole cell lysate (arrowhead).
Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 1,350.
Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDye™ 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SLIT2
Entrez GeneID
9353Protein Accession#
O94813;NP_004778Gene Name
SLIT2
Gene Alias
FLJ14420, SLIL3, Slit-2
Gene Description
slit homolog 2 (Drosophila)
Omim ID
603746Gene Ontology
HyperlinkOther Designations
slit homolog 2
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Interactome
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Pathway
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Disease
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Publication Reference
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SLIT2-mediated ROBO2 signaling restricts kidney induction to a single site.
Grieshammer U, Le Ma, Plump AS, Wang F, Tessier-Lavigne M, Martin GR.
Developmental Cell 2004 May; 6(5):709.
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Slit protein-mediated inhibition of CXCR4-induced chemotactic and chemoinvasive signaling pathways in breast cancer cells.
Prasad A, Fernandis AZ, Rao Y, Ganju RK.
The Journal of Biological Chemistry 2004 Mar; 279(10):9115.
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Frequent epigenetic inactivation of the SLIT2 gene in gliomas.
Dallol A, Krex D, Hesson L, Eng C, Maher ER, Latif F.
Oncogene 2003 Jul; 22(29):4611.
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SLIT2-mediated ROBO2 signaling restricts kidney induction to a single site.
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