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Last updated: 2016/12/4
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SLIT2 polyclonal antibody

  • Catalog # : PAB11259
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SLIT2.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 484-500 of human SLIT2.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chicken, Clawed frog, Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:1500-1:6000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using SLIT2 polyclonal antibody (Cat # PAB11259) shows detection of a band at ~165 KDa (Lane 1) corresponding to SLIT2 present in a chicken spinal cord whole cell lysate (arrowhead).
    Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1 : 1,350.
    Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).
    Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
    IRDye™ 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 9353
  • Protein Accession#:
  • O94813;NP_004778
  • Gene Name:
  • SLIT2
  • Gene Alias:
  • FLJ14420,SLIL3,Slit-2
  • Gene Description:
  • slit homolog 2 (Drosophila)
  • Other Designations:
  • slit homolog 2
  • Gene Pathway
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