ROBO1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ROBO1.
Immunogen
A synthetic peptide corresponding to amino acids 1632-1644 of human ROBO1.
Host
Rabbit
Reactivity
Dog, Human, Mouse, Rat
Specificity
This affinity purified antibody is directed against human ROBO-1 protein. Cross reactivity will occur with all isoforms of ROBO-1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:30000-1:160000)
Western Blot (1:500-1:3000)
Immunohistochemistry (2-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using ROBO1 polyclonal antibody (Cat # PAB11258) shows detection of a band at ~181 kDa correspond-ing to ROBO1 present in mouse brain lysate (arrowhead).
Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and probed with the primary antibody diluted to 1:1,000.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of ROBO1 polyclonal antibody (Cat # PAB11258) was used at a concentration of 5 ug/mL to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows staining of human brain tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ROBO1
Entrez GeneID
6091Protein Accession#
Q9Y6N7;NP_002932(isoforma)Gene Name
ROBO1
Gene Alias
DUTT1, FLJ21882, MGC131599, MGC133277, SAX3
Gene Description
roundabout, axon guidance receptor, homolog 1 (Drosophila)
Omim ID
602430Gene Ontology
HyperlinkGene Summary
Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
axon guidance receptor|roundabout 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Induction of tumor angiogenesis by Slit-Robo signaling and inhibition of cancer growth by blocking Robo activity.
Wang B, Xiao Y, Ding BB, Zhang N, Yuan X, Gui L, Qian KX, Duan S, Chen Z, Rao Y, Geng JG.
Cancer Cell 2003 Jul; 4(1):19.
Application:IHC-P, WB, Human, Mouse, HEK 293 cells, HUVECs, Mouse tumors.
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Quantification of expression of netrins, slits and their receptors in human prostate tumors.
Latil A, Chene L, Cochant-Priollet B, Mangin P, Fournier G, Berthon P, Cussenot O.
International Journal of Cancer 2003 Jan; 103(3):306.
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Robo1 and Robo2 are homophilic binding molecules that promote axonal growth.
Hivert B, Liu Z, Chuang CY, Doherty P, Sundaresan V.
Molecular and Cellular Neurosciences 2002 Dec; 21(4):534.
Application:IF, WB-Tr, Mouse, 3T3, Retinal ganglion cells.
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Induction of tumor angiogenesis by Slit-Robo signaling and inhibition of cancer growth by blocking Robo activity.
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