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Last updated: 2018/1/21
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ROBO1 polyclonal antibody

  • Catalog # : PAB11258
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ROBO1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 1632-1644 of human ROBO1.
  • Host:
  • Rabbit
  • Reactivity:
  • Dog, Human, Mouse, Rat
  • Specificity:
  • This affinity purified antibody is directed against human ROBO-1 protein. Cross reactivity will occur with all isoforms of ROBO-1.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:30000-1:160000)
    Western Blot (1:500-1:3000)
    Immunohistochemistry (2-10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using ROBO1 polyclonal antibody (Cat # PAB11258) shows detection of a band at ~181 kDa correspond-ing to ROBO1 present in mouse brain lysate (arrowhead).
    Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and probed with the primary antibody diluted to 1:1,000.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of ROBO1 polyclonal antibody (Cat # PAB11258) was used at a concentration of 5 ug/mL to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
    This image shows staining of human brain tissue.
    Tissue was formalin-fixed and paraffin embedded.
    Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
  • Immunofluorescence
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Gene Information
  • Entrez GeneID:
  • 6091
  • Protein Accession#:
  • Q9Y6N7;NP_002932(isoforma)
  • Gene Name:
  • ROBO1
  • Gene Alias:
  • DUTT1,FLJ21882,MGC131599,MGC133277,SAX3
  • Gene Description:
  • roundabout, axon guidance receptor, homolog 1 (Drosophila)
  • Gene Summary:
  • Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • axon guidance receptor,roundabout 1
  • Gene Pathway
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