RNF34 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot using RNF34 polyclonal antibody (Cat # PAB11257) shows detection of human RNF34 (arrowhead) in lysate.
Lanes corresponding to empty vector 293T cell lysate (mock, lane 1) ; RNF34 transfected lysate (Lane 2) and RFFL transfected lysate (Lane 3), are shown using 20 ug of lysate per lane.
Lysates were prepared from equivalent numbers of cells.
The primary antibody diluted to 1:1,000.
Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant RNF34.
Immunogen
Recombinant protein corresponding to amino acids 1-373 of human RNF34.
Host
Rabbit
Reactivity
Bovine, Dog, Human, Mouse, Orangutan, Rat
Specificity
This protein A purified antibody is directed against human RNF34 protein. Expect reactivity with isoform 1 and 2 of RNF34.
Form
Lyophilized
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:4000-1:20000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot after reconstitution to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using RNF34 polyclonal antibody (Cat # PAB11257) shows detection of human RNF34 (arrowhead) in lysate.
Lanes corresponding to empty vector 293T cell lysate (mock, lane 1) ; RNF34 transfected lysate (Lane 2) and RFFL transfected lysate (Lane 3), are shown using 20 ug of lysate per lane.
Lysates were prepared from equivalent numbers of cells.
The primary antibody diluted to 1:1,000.
Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — RNF34
Entrez GeneID
80196Protein Accession#
NP_919247;Q969K3Gene Name
RNF34
Gene Alias
FLJ21786, RFI, RIF, RIFF
Gene Description
ring finger protein 34
Omim ID
608299Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
FYVE-RING finger protein MOMO|RING finger protein RIFF
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Interactome
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Publication Reference
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Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer.
Sasaki S, Nakamura T, Arakawa H, Mori M, Watanabe T, Nagawa H, Croce CM.
Oncogene 2002 Jul; 21(32):5024.
Application：IF, WB-Tr, Human, HeLa, Jurkat cells.
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Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer.
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