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Last updated: 2016/12/4
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RNF34 polyclonal antibody

  • Catalog # : PAB11257
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant RNF34.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-373 of human RNF34.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Dog, Human, Mouse, Orangutan, Rat
  • Specificity:
  • This protein A purified antibody is directed against human RNF34 protein. Expect reactivity with isoform 1 and 2 of RNF34.
  • Form:
  • Lyophilized
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:4000-1:20000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot after reconstitution to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using RNF34 polyclonal antibody (Cat # PAB11257) shows detection of human RNF34 (arrowhead) in lysate.
    Lanes corresponding to empty vector 293T cell lysate (mock, lane 1) ; RNF34 transfected lysate (Lane 2) and RFFL transfected lysate (Lane 3), are shown using 20 ug of lysate per lane.
    Lysates were prepared from equivalent numbers of cells.
    The primary antibody diluted to 1:1,000.
    Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.
  • Immunofluorescence
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunofluorescence
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_919247;Q969K3
  • Gene Name:
  • RNF34
  • Gene Alias:
  • FLJ21786,RFI,RIF,RIFF
  • Gene Description:
  • ring finger protein 34
  • Gene Summary:
  • The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
  • Other Designations:
  • FYVE-RING finger protein MOMO,RING finger protein RIFF
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