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Last updated: 2017/11/19
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RNF25 polyclonal antibody

  • Catalog # : PAB11256
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of RNF25.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human RNF25.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:40000)
    Western Blot (1:200-1:2000)
    Immunoprecipitation (1 ug)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using RNF25 polyclonal antibody (Cat # PAB11256) shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 3 is a vector only control. The primary antibody diluted to 1 : 1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
  • Immunoprecipitation
  • Immunoprecipitation
  • 1 ug of RNF25 polyclonal antibody (Cat # PAB11256) was used in immunoprecipitation of 20 ug HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_071898;Q96BH1
  • Gene Name:
  • RNF25
  • Gene Alias:
  • AO7,FLJ13906
  • Gene Description:
  • ring finger protein 25
  • Gene Summary:
  • The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination. [provided by RefSeq
  • Other Designations:
  • ring finger protein AO7
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