RNF25 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using RNF25 polyclonal antibody (Cat # PAB11256) shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 3 is a vector only control. The primary antibody diluted to 1 : 1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
Immunoprecipitation
1 ug of RNF25 polyclonal antibody (Cat # PAB11256) was used in immunoprecipitation of 20 ug HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RNF25.
Immunogen
A synthetic peptide corresponding to C-terminus of human RNF25.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:40000)
Western Blot (1:200-1:2000)
Immunoprecipitation (1 ug)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using RNF25 polyclonal antibody (Cat # PAB11256) shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 3 is a vector only control. The primary antibody diluted to 1 : 1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.Immunoprecipitation
1 ug of RNF25 polyclonal antibody (Cat # PAB11256) was used in immunoprecipitation of 20 ug HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RNF25
Entrez GeneID
64320Protein Accession#
NP_071898;Q96BH1Gene Name
RNF25
Gene Alias
AO7, FLJ13906
Gene Description
ring finger protein 25
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination. [provided by RefSeq
Other Designations
ring finger protein AO7
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Interactome
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Publication Reference
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RING finger protein AO7 supports NF-kappaB-mediated transcription by interacting with the transactivation domain of the p65 subunit.
Asamitsu K, Tetsuka T, Kanazawa S, Okamoto T.
The Journal of Biological Chemistry 2003 Jul; 278(29):26879.
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RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination.
Lorick KL, Jensen JP, Fang S, Ong AM, Hatakeyama S, Weissman AM.
PNAS 1999 Sep; 96(20):11364.
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RING finger protein AO7 supports NF-kappaB-mediated transcription by interacting with the transactivation domain of the p65 subunit.
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