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RFFL polyclonal antibody

  • Catalog # : PAB11254
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant RFFL.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-363 of human RFFL.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Dog, Human, Mouse, Rat
  • Specificity:
  • This protein A purified antibody is directed against human RFFL protein. Expect reactivity with isoform 1, 2 and 3 of RFFL.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:4000-1:20000)
    Western Blot (1:500-1:3000)
    Immunohistochemistry (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using RFFL polyclonal antibody (Cat # PAB11254) shows detection of RFFL (arrowhead) in lysate.
    Lanes correspond to empty vector 293T cell lysate (1) ; RNF34 transfected lysate (2) and RFFL transfected lysate (3), are shown using 20 uL of lysate per lane.
    The primary antibody diluted to 1:1,000.
    Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of RFFL polyclonal antibody (Cat # PAB11254) shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue.
    Tissue was formalin-fixed and paraffin embedded.
    Brown color indicates presence of protein, blue color shows cell nuclei.
    Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
  • ELISA
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_476519;Q8WZ73
  • Gene Name:
  • RFFL
  • Gene Alias:
  • RIFIFYLIN,RNF189,RNF34L
  • Gene Description:
  • ring finger and FYVE-like domain containing 1
  • Other Designations:
  • FYVE-RING finger protein SAKURA,fring,rififylin
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