Western blot using RFFL polyclonal antibody (Cat # PAB11254) shows detection of RFFL (arrowhead) in lysate. Lanes correspond to empty vector 293T cell lysate (1) ; RNF34 transfected lysate (2) and RFFL transfected lysate (3), are shown using 20 uL of lysate per lane. The primary antibody diluted to 1:1,000. Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.
Immunohistochemistry of RFFL polyclonal antibody (Cat # PAB11254) shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.