RFFL polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Western blot using RFFL polyclonal antibody (Cat # PAB11254) shows detection of RFFL (arrowhead) in lysate.
Lanes correspond to empty vector 293T cell lysate (1) ; RNF34 transfected lysate (2) and RFFL transfected lysate (3), are shown using 20 uL of lysate per lane.
The primary antibody diluted to 1:1,000.
Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of RFFL polyclonal antibody (Cat # PAB11254) shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
-
Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant RFFL.
Immunogen
Recombinant protein corresponding to amino acids 1-363 of human RFFL.
Host
Rabbit
Reactivity
Bovine, Dog, Human, Mouse, Rat
Specificity
This protein A purified antibody is directed against human RFFL protein. Expect reactivity with isoform 1, 2 and 3 of RFFL.
Form
Lyophilized
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:4000-1:20000)
Western Blot (1:500-1:3000)
Immunohistochemistry (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.02 M KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Western blot using RFFL polyclonal antibody (Cat # PAB11254) shows detection of RFFL (arrowhead) in lysate.
Lanes correspond to empty vector 293T cell lysate (1) ; RNF34 transfected lysate (2) and RFFL transfected lysate (3), are shown using 20 uL of lysate per lane.
The primary antibody diluted to 1:1,000.
Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of RFFL polyclonal antibody (Cat # PAB11254) shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — RFFL
Entrez GeneID
117584Protein Accession#
NP_476519;Q8WZ73Gene Name
RFFL
Gene Alias
RIFIFYLIN, RNF189, RNF34L
Gene Description
ring finger and FYVE-like domain containing 1
Omim ID
609735Gene Ontology
HyperlinkOther Designations
FYVE-RING finger protein SAKURA|fring|rififylin
-
Interactome
-
Disease
-
Publication Reference
-
Crystal structure of a FYVE-type zinc finger domain from the caspase regulator CARP2.
Tibbetts MD, Shiozaki EN, Gu L, McDonald ER 3rd, El-Deiry WS, Shi Y.
Structure 2004 Dec; 12(12):2257.
-
Over-expression of Rififylin, a new RING finger and FYVE-like domain-containing protein, inhibits recycling from the endocytic recycling compartment.
Coumailleau F, Das V, Alcover A, Raposo G, Vandormael-Pournin S, Le Bras S, Baldacci P, Dautry-Varsat A, Babinet C, Cohen-Tannoudji M.
Molecular Biology of the Cell 2004 Oct; 15(10):4444.
Application：WB, Human, HeLa cells.
-
Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth.
McDonald ER 3rd, El-Deiry WS.
PNAS 2004 Apr; 101(16):6170.
-
Crystal structure of a FYVE-type zinc finger domain from the caspase regulator CARP2.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com