PRKCB polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using PRKCB polyclonal antibody (Cat # PAB11251) shows detection of PRKCB in ~25 ug of U251 whole cell lysate (glioma derived).
The arrowhead corresponds to 76 kDa PRKCB.
The primary antibody at a 1:1,000 dilution.
Personal Communication, Howard Fine and Svetlana Kotliarova, CCR-NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKCB.
Immunogen
A synthetic peptide corresponding to internal region of human PRKCB.
Host
Rabbit
Reactivity
Bovine, Human, Monkey, Mouse, Rabbit, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:75000-1:80000)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using PRKCB polyclonal antibody (Cat # PAB11251) shows detection of PRKCB in ~25 ug of U251 whole cell lysate (glioma derived).
The arrowhead corresponds to 76 kDa PRKCB.
The primary antibody at a 1:1,000 dilution.
Personal Communication, Howard Fine and Svetlana Kotliarova, CCR-NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKCB
Entrez GeneID
5579Protein Accession#
NP_997700.1;P05771Gene Name
PRKCB
Gene Alias
MGC41878, PKC-beta, PKCB, PRKCB1, PRKCB2
Gene Description
protein kinase C, beta
Omim ID
176970Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
protein kinase C, beta 1 polypeptide
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Interactome
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Pathway
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Disease
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Publication Reference
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PKC{alpha} suppresses osteoblastic differentiation.
Nakura A, Higuchi C, Yoshida K, Yoshikawa H.
Bone 2011 Mar; 48(3):476.
Application：WB-Ce, Mouse, MC3T3-E1 cells.
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The protein kinase Cbeta-selective inhibitor, Enzastaurin (LY317615.HCl), suppresses signaling through the AKT pathway, induces apoptosis, and suppresses growth of human colon cancer and glioblastoma xenografts.
Graff JR, McNulty AM, Hanna KR, Konicek BW, Lynch RL, Bailey SN, Banks C, Capen A, Goode R, Lewis JE, Sams L, Huss KL, Campbell RM, Iversen PW, Neubauer BL, Brown TJ, Musib L, Geeganage S, Thornton D.
Cancer Research 2005 Aug; 65(16):7462.
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Protein kinase C alpha/beta inhibitor Go6976 promotes formation of cell junctions and inhibits invasion of urinary bladder carcinoma cells.
Koivunen J, Aaltonen V, Koskela S, Lehenkari P, Laato M, Peltonen J.
Cancer Research 2004 Aug; 64(16):5693.
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Characterization of site-specific mutants altered at protein kinase C beta 1 isozyme autophosphorylation sites.
Zhang J, Wang L, Petrin J, Bishop WR, Bond RW.
PNAS 1993 Jul; 90(13):6130.
Application：WB-Ce, WB-Tr, Monkey, COS cells.
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PKC{alpha} suppresses osteoblastic differentiation.
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