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Last updated: 2016/10/23
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PPARG polyclonal antibody

  • Catalog # : PAB11248
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PPARG.
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus of human PPARG.
  • Host:
  • Rabbit
  • Reactivity:
  • Dog, Human, Macaque, Mouse, Pig
  • Specificity:
  • This affinity purified antibody is directed against human PPAR gamma isoform 2 protein. No reactivity is expected against other subtypes of PPAR.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:4000-1:40000)
    Western Blot (1:1000-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using PPARG polyclonal antibody (Cat # PAB11248) shows detection of PPARG protein in a mouse NIH/3T3 whole cell lysate (Lane 1 arrowhead).
    Approximately 20 ug of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose.
    Primary antibody was used at a 1 : 2,000 dilution in 5% BLOTTO PBS solution.
    The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye.
    IgG™800 Conjugated Affinity Purified Goat-anti-Rabbit [H&L] MX10.
    Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (Lane M).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5468
  • Protein Accession#:
  • P37231;NP_056953
  • Gene Name:
  • Gene Alias:
  • Gene Description:
  • peroxisome proliferator-activated receptor gamma
  • Gene Summary:
  • This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000185030,OTTHUMP00000185033,OTTHUMP00000185037,PPAR gamma,nuclear receptor subfamily 1 group C member 3,peroxisome proliferative activated receptor gamma,peroxisome proliferative activated receptor, gamma,peroxisome proliferator activated recept
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