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Last updated: 2017/10/15

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POLK polyclonal antibody

  • Catalog # : PAB11246
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of POLK.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 817-830 of human POLK.
  • Host:
  • Goat
  • Reactivity:
  • Chimpanzee, Human, Mouse, Orangutan
  • Specificity:
  • Expect reactivity with multiple splice variants of this protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000-1:10000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using POLK polyclonal antibody (Cat # PAB11246) shows detection ofa band ~98 KDa corresponding to human POLK (arrowhead).
    Approximately 35 ug of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed withthe primary antibody diluted to 1 : 1,000.
    Reaction occurred overnight at 4°C followed bywashes and reaction with a 1 : 20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at roomtemperature.
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDyeis a trademark of LI-COR, Inc.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • POLK
  • Gene Alias:
  • DINB1,DINP,POLQ
  • Gene Description:
  • polymerase (DNA directed) kappa
  • Other Designations:
  • DINB protein,DNA polymerase kappa,other name:POLK,polymerase (DNA-directed) kappa
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