POLK polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using POLK polyclonal antibody (Cat # PAB11246) shows detection ofa band ~98 KDa corresponding to human POLK (arrowhead).
Approximately 35 ug of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed withthe primary antibody diluted to 1 : 1,000.
Reaction occurred overnight at 4°C followed bywashes and reaction with a 1 : 20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at roomtemperature.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDyeis a trademark of LI-COR, Inc.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of POLK.
Immunogen
A synthetic peptide corresponding to amino acids 817-830 of human POLK.
Host
Goat
Reactivity
Chimpanzee, Human, Mouse, Orangutan
Specificity
Expect reactivity with multiple splice variants of this protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using POLK polyclonal antibody (Cat # PAB11246) shows detection ofa band ~98 KDa corresponding to human POLK (arrowhead).
Approximately 35 ug of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed withthe primary antibody diluted to 1 : 1,000.
Reaction occurred overnight at 4°C followed bywashes and reaction with a 1 : 20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at roomtemperature.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDyeis a trademark of LI-COR, Inc.Enzyme-linked Immunoabsorbent Assay
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Gene Info — POLK
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Interactome
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Disease
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Publication Reference
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Translesion synthesis past 2'-deoxyxanthosine, a nitric oxide-derived DNA adduct, by mammalian DNA polymerases.
Yasui M, Suzuki N, Miller H, Matsuda T, Matsui S, Shibutani S.
Journal of Molecular Biology 2004 Nov; 344(3):665.
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Crystal structure of the catalytic core of human DNA polymerase kappa.
Uljon SN, Johnson RE, Edwards TA, Prakash S, Prakash L, Aggarwal AK.
Structure 2004 Aug; 12(8):1395.
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A mechanism for the exclusion of low-fidelity human Y-family DNA polymerases from base excision repair.
Haracska L, Prakash L, Prakash S.
Genes & Development 2003 Nov; 17(22):2777.
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Translesion synthesis past 2'-deoxyxanthosine, a nitric oxide-derived DNA adduct, by mammalian DNA polymerases.
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