Western blot using PNKP polyclonal antibody (Cat # PAB11245) shows detection of a 57 kDa band corresponding to : Lane 1, human PNKP in a Y190 yeast cell lysate. Lane 2, Y190 yeast cell lysate + human PNKP (Gal DNA BP). Lane 3, EM9 XH Chinese hamster ovary cell lysate. Lane 4, EM9 XH Chinese hamster ovary cell lysate + human PNK. Lane 5, a HeLa cell lysate. Approximately 10 ug of lysate was run on SDS-PAGE and reaction with a 1:1000 dilution of PNKP polyclonal antibody.