PNKP polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot using PNKP polyclonal antibody (Cat # PAB11245) shows detection of a 57 kDa band corresponding to :
Lane 1, human PNKP in a Y190 yeast cell lysate.
Lane 2, Y190 yeast cell lysate + human PNKP (Gal DNA BP).
Lane 3, EM9 XH Chinese hamster ovary cell lysate.
Lane 4, EM9 XH Chinese hamster ovary cell lysate + human PNK.
Lane 5, a HeLa cell lysate.
Approximately 10 ug of lysate was run on SDS-PAGE and reaction with a 1:1000 dilution of PNKP polyclonal antibody.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PNKP.
Immunogen
A synthetic peptide corresponding to internal region of human PNKP.
Host
Rabbit
Reactivity
Chimpanzee, Dog, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:3000-1:12000)
Western Blot (1:300-1:1500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using PNKP polyclonal antibody (Cat # PAB11245) shows detection of a 57 kDa band corresponding to :
Lane 1, human PNKP in a Y190 yeast cell lysate.
Lane 2, Y190 yeast cell lysate + human PNKP (Gal DNA BP).
Lane 3, EM9 XH Chinese hamster ovary cell lysate.
Lane 4, EM9 XH Chinese hamster ovary cell lysate + human PNK.
Lane 5, a HeLa cell lysate.
Approximately 10 ug of lysate was run on SDS-PAGE and reaction with a 1:1000 dilution of PNKP polyclonal antibody.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PNKP
Entrez GeneID
11284Protein Accession#
Q96T60;NP_009185Gene Name
PNKP
Gene Alias
PNK
Gene Description
polynucleotide kinase 3'-phosphatase
Omim ID
605610Gene Ontology
HyperlinkOther Designations
Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP)|Polynucleotide kinase 3'-phosphatase, amino acids 68-521|Polynucleotide kinase 3'-phosphatase, partial sequence|polynucleotide kinase 3-prime-phosphatase
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Interactome
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Disease
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Publication Reference
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Spectroscopic studies of DNA and ATP binding to human polynucleotide kinase: evidence for a ternary complex.
Mani RS, Karimi-Busheri F, Fanta M, Cass CE, Weinfeld M.
Biochemistry 2003 Oct; 42(41):12077.
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Involvement of human polynucleotide kinase in double-strand break repair by non-homologous end joining.
Chappell C, Hanakahi LA, Karimi-Busheri F, Weinfeld M, West SC.
The EMBO Journal 2002 Jun; 21(11):2827.
Application：Immunodepletion, Human, GM00558 cells.
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Molecular characterization of a human DNA kinase.
Karimi-Busheri F, Daly G, Robins P, Canas B, Pappin DJ, Sgouros J, Miller GG, Fakhrai H, Davis EM, Le Beau MM, Weinfeld M.
The Journal of Biological Chemistry 1999 Aug; 274(34):24187.
Application：IF, Human, A-549 cells.
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Spectroscopic studies of DNA and ATP binding to human polynucleotide kinase: evidence for a ternary complex.
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