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Last updated: 2017/4/30
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PIN1 polyclonal antibody

  • Catalog # : PAB11244
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PIN1.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human PIN1.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Dog, Human, Monkey, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2500-1:10000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using PIN1 polyclonal antibody (Cat # PAB11244) to detect endogenous PIN1 in HeLa whole cell lysates.
    The sample was run in duplicate.
    A band representing PIN1 is indicated by the arrowhead.
    Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose.
    The membrane was probed with the primary antibody at a 1 : 700 dilution.
    A 1 : 5,000 dilution of HRP Gt-a-Rabbit IgG was used with a 15 sec exposure time.
    Personal Communication, L. D'agostino and A. Giordano, SHRO, Philadelphia, PA.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5300
  • Protein Accession#:
  • NP_006212;Q13526
  • Gene Name:
  • PIN1
  • Gene Alias:
  • DOD,UBL5
  • Gene Description:
  • peptidylprolyl cis/trans isomerase, NIMA-interacting 1
  • Gene Summary:
  • The human PIN1 gene encodes an essential nuclear peptidylprolyl cis-trans isomerase (PPIase; EC 5.2.1.8) involved in regulation of mitosis. PIN1 belongs to a class of PPIases that includes the E. coli parvulin, yeast Ess1, and Drosophila dodo (dod) gene products (Lu et al., 1996 [PubMed 8606777]).[supplied by OMIM
  • Other Designations:
  • peptidyl-prolyl cis/trans isomerase, NIMA-interacting,prolyl isomerase,protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1,protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1
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