PIN1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using PIN1 polyclonal antibody (Cat # PAB11244) to detect endogenous PIN1 in HeLa whole cell lysates.
The sample was run in duplicate.
A band representing PIN1 is indicated by the arrowhead.
Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose.
The membrane was probed with the primary antibody at a 1 : 700 dilution.
A 1 : 5,000 dilution of HRP Gt-a-Rabbit IgG was used with a 15 sec exposure time.
Personal Communication, L. D'agostino and A. Giordano, SHRO, Philadelphia, PA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PIN1.
Immunogen
A synthetic peptide corresponding to internal region of human PIN1.
Host
Rabbit
Reactivity
Bovine, Dog, Human, Monkey, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2500-1:10000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using PIN1 polyclonal antibody (Cat # PAB11244) to detect endogenous PIN1 in HeLa whole cell lysates.
The sample was run in duplicate.
A band representing PIN1 is indicated by the arrowhead.
Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose.
The membrane was probed with the primary antibody at a 1 : 700 dilution.
A 1 : 5,000 dilution of HRP Gt-a-Rabbit IgG was used with a 15 sec exposure time.
Personal Communication, L. D'agostino and A. Giordano, SHRO, Philadelphia, PA.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — PIN1
Entrez GeneID
5300Protein Accession#
NP_006212;Q13526Gene Name
PIN1
Gene Alias
DOD, UBL5
Gene Description
peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Omim ID
601052Gene Ontology
HyperlinkGene Summary
The human PIN1 gene encodes an essential nuclear peptidylprolyl cis-trans isomerase (PPIase; EC 5.2.1.8) involved in regulation of mitosis. PIN1 belongs to a class of PPIases that includes the E. coli parvulin, yeast Ess1, and Drosophila dodo (dod) gene products (Lu et al., 1996 [PubMed 8606777]).[supplied by OMIM
Other Designations
peptidyl-prolyl cis/trans isomerase, NIMA-interacting|prolyl isomerase|protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1|protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1
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Interactome
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Disease
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Publication Reference
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The peptidyl-prolyl isomerase Pin1 regulates granulocyte-macrophage colony-stimulating factor mRNA stability in T lymphocytes.
Esnault S, Shen ZJ, Whitesel E, Malter JS.
Journal of Immunology 2006 Nov; 177(10):6999.
Application：IP, WB, Human, HeLa cells, PBMCs, T lymphocytes.
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Aberrant expression of beta-catenin, Pin1 and cylin D1 in salivary adenoid cystic carcinoma: relation to tumor proliferation and metastasis.
Zhou CX, Gao Y.
Oncology Reports 2006 Sep; 16(3):505.
Application：IHC-P, WB-Ce, Human, ACC-2, ACC-M, SACC83, SACCLM (LM) cells, Human salivary adenoid cystic carcinomas.
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Prolyl isomerase Pin1 expression predicts prognosis in patients with esophageal squamous cell carcinoma and correlates with cyclinD1 expression.
Fukuchi M, Fukai Y, Kimura H, Sohda M, Miyazaki T, Nakajima M, Masuda N, Tsukada K, Kato H, Kuwano H.
International Journal of Oncology 2006 Aug; 29(2):329.
Application：IHC-P, WB, Human, Human esophageal squamous cell carcinoma, Human esophageal squamous carcinoma-derived cell lines.
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The peptidyl-prolyl isomerase Pin1 regulates granulocyte-macrophage colony-stimulating factor mRNA stability in T lymphocytes.
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