Goat Anti-Rat IgG F(ab')2 secondary antibody (Phycoerythrin)
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Specification
Product Description
Goat anti-rat IgG recognizes rat IgG F(ab')2 fragment. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Phycoerythrin.
Immunogen
Rat IgG F(ab')2 fragment
Host
Goat
Reactivity
Rat
Form
Lyophilized
Conjugation
R-Phycoerythrin (RPE)
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
Lyophilized from 0.01 M Na2HPO4, 250 mM NaCl, pH 7.6 (0.05% (w/v) sodium azide, 10 mg/mL BSA (Immunoglobulin and Protease free)).
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
Minimum cross reactivity to bovine, horse, human.
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Applications
Immunofluorescence
Flow Cytometry
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Publication Reference
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CD44 Antibody Inhibition of Macrophage Phagocytosis Targets Fcγ Receptor- and Complement Receptor 3-Dependent Mechanisms.
Amash A, Wang L, Wang Y, Bhakta V, Fairn GD, Hou M, Peng J, Sheffield WP, Lazarus AH.
The Journal of Immunology 2016 Apr; 196(8):3331.
Application：Flow Cyt, IF, Mouse, Peritoneal macrophages, RAW264.7 cells.
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CD44 Antibody Inhibition of Macrophage Phagocytosis Targets Fcγ Receptor- and Complement Receptor 3-Dependent Mechanisms.
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