Goat Anti-Rat IgG F(ab')2 secondary antibody (Fluorescein)
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Specification
Product Description
Goat anti-rat IgG recognizes rat IgG F(ab')2 fragment. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Fluorescein.
Immunogen
Rat IgG F(ab')2 fragment
Host
Goat
Reactivity
Rat
Specificity
No reaction was observed against rat IgG F(c), anti-Pepsin, anti-goat IgG F(c) or bovine, horse and human serum proteins.
Form
Lyophilized
Conjugation
FITC
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation Note
2.4 moles FITC per mole of goat IgG F(ab')2
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
Lyophilized from 5 mM sodium phosphate, 125 mM NaCl, 50% (v/v) glycerol, pH 7.6 (0.05% (w/v) sodium azide, 15 mg/mL BSA (Immunoglobulin and Protease free))
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
Minimum cross reactivity to bovine, horse, human.
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Applications
Immunofluorescence
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