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Last updated: 2017/3/26
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FOXO1 (phospho S319) polyclonal antibody

  • Catalog # : PAB10970
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of FOXO1.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S319 of human FOXO1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 78-82
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Immunoaffinity purification
  • Concentration:
  • 1 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extracts from NIH/3T3 cells untreated (Lane 1) or treated (Lane 2) with serum starvation using FFOXO1 (phospho S319) polyclonal antibody (Cat # PAB10970).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embbeded human breast carcinoma tissue using FFOXO1 (phospho S319) polyclonal antibody (Cat # PAB10970) (A) or preincubated with blocking peptide (B).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of manthanol-fixed HeLa cells using FOXO1 (phospho S319) polyclonal antibody (Cat # PAB10970).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2308
  • Gene Name:
  • FOXO1
  • Gene Alias:
  • FKH1,FKHR,FOXO1A
  • Gene Description:
  • forkhead box O1
  • Gene Summary:
  • This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000018301,forkhead box O1A (rhabdomyosarcoma),forkhead homolog in rhabdomyosarcoma,forkhead, Drosophila, homolog of, in rhabdomyosarcoma
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