Sheep Anti-Rat IgG (H&L) secondary antibody (Peroxidase)
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Specification
Product Description
Sheep anti-rat IgG recognizes rat IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Peroxidase.
Immunogen
Rat IgG whole molecule
Host
Sheep
Reactivity
Rat
Form
Liquid
Conjugation
HRP
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation Note
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 10 mM potassium phosphate, 75 mM NaCl, 50% (v/v) glycerol, pH 7.2 (0.01% (w/v) gentamicin sulfate (Do NOT add sodium azide!), 10 mg/mL BSA (Immunoglobulin and Protease free))
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
Dot Blot
Enzyme Immunoassay
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Publication Reference
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Immunohistochemistry with enzyme labeled antibodies: a brief review.
Farr AG, Nakane PK.
J Immunol Methods 1981 Jan; 47(2):129.
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Immunohistochemistry with enzyme labeled antibodies: a brief review.
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