Rabbit Anti-Rat IgG (H&L) secondary antibody (Fluorescein)
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Specification
Product Description
Rabbit anti-rat IgG recognizes rat IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Fluorescein.
Immunogen
Rat IgG whole molecule
Host
Rabbit
Reactivity
Rat
Form
Lyophilized
Conjugation
FITC
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation Note
3.1 moles FITC per mole of rabbit IgG
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
Lyophilized from 10 mM potassium phosphate, 75 mM NaCl, 50% (v/v) glycerol, pH 7.2 (0.01% (w/v) sodium azide, 10 mg/mL BSA (ImmunoglobulLyophilized from and Protease free))
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Flow Cytometry
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Publication Reference
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Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.
The TH, Feltkamp TE.
Immunology 1970 Jun; 18(6):865.
Application：Conjugation, Fluorescein isothiocyanate (FITC).
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Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.
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