Sheep Anti-Rabbit IgG (H&L) secondary antibody (Fluorescein)
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Specification
Product Description
Sheep anti-rabbit IgG recognizes rabbit IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Fluorescein.
Immunogen
Rabbit IgG whole molecule
Host
Sheep
Reactivity
Rabbit
Form
Liquid
Conjugation
FITC
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation Note
2.7 moles FITC per mole of sheep IgG
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 10 mM potassium phosphate, 75 mM NaCl, 50% (v/v) glycerol, pH 7.2 (0.01% (w/v) sodium azide, 10 mg/mL BSA (Immunoglobulin and Protease free))
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Flow Cytometry
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Publication Reference
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Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.
The TH, Feltkamp TE.
Immunology 1970 Jun; 18(6):865.
Application：Conjugation, Fluorescein isothiocyanate (FITC).
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Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.
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