Goat Anti-Rabbit IgG F(ab')2 secondary antibody (Peroxidase)
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Specification
Product Description
Goat anti-rabbit IgG recognizes rabbit IgG F(ab')2 fragment. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Peroxidase.
Immunogen
Rabbit IgG F(ab')2 fragment
Host
Goat
Reactivity
Rabbit
Form
Lyophilized
Conjugation
HRP
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation Note
Recommend Usage
ELISA (1:10000-1:50000)
Immunohistochemistry (1:500-1:2500)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.01 M sodium phosphate, 0.15 M sodium chloride, pH 7.2 (0.01% (w/v) gentamicin sulfate (Do NOT add sodium azide!), 10 mg/mL BSA (Immunoglobulin and Protease free)).
Restore with 1 mL deionized water (or equivalent).Storage Instruction
Store at 4°C prior to restoration.
For extended storage aliquot contents and freeze at -20°C or below.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
Dot Blot
Enzyme Immunoassay
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Publication Reference
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Immunohistochemistry with enzyme labeled antibodies: a brief review.
Farr AG, Nakane PK.
J Immunol Methods 1981 Jan; 47(2):129.
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Immunohistochemistry with enzyme labeled antibodies: a brief review.
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