Goat Anti-Rabbit IgG F(ab')2 secondary antibody (Fluorescein)
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Specification
Product Description
Goat anti-rabbit IgG recognizes rabbit IgG F(ab')2 fragment. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Fluorescein.
Immunogen
Rabbit IgG F(ab')2 fragment
Host
Goat
Reactivity
Rabbit
Form
Lyophilized
Conjugation
FITC
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Isotype
IgG
Conjugation Note
5.3 moles Fluorescein (FITC) per mole of IgG
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 (0.01% (w/v) thimerosal, 10 mg/mL BSA (Immunoglobulin and Protease free))
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Flow Cytometry
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Publication Reference
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Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG.
D Blakeslee, M G Baines.
Journal of Immunological Methods 1976 Dec; 13(3-4):305.
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Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG.
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