Goat Anti-Rabbit IgG (H&L) secondary antibody (Fluorescein)
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Specification
Product Description
Goat anti-rabbit IgG recognizes rabbit IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Fluorescein.
Immunogen
Rabbit IgG whole molecule
Host
Goat
Reactivity
Rabbit
Specificity
IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Form
Lyophilized
Conjugation
FITC
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Isotype
IgG
Conjugation Note
FP Value : 0.85 moles Fluorescein (FITC) per mole of IgG
Recommend Usage
FLISA (1:10000-1:50000)
Flow Cytometry (1:500-1:2500)
Immunofluorescence (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM potassium phosphate, 150 mM NaCl (10 mg/mL bovine serum albumin (BSA), 0.01% (w/v) sodium azide).
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 1 mL of deionized (sterilized) water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Flow Cytometry
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Publication Reference
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Hsa_circ_0016760 exacerbates the malignant development of non‑small cell lung cancer by sponging miR‑145‑5p/FGF5.
Zheng Zhu, Qiyong Wu, Ming Zhang, Jichun Tong, Bin Zhong, Kai Yuan.
Oncology Reports 2020 Dec; 45(2):501.
Application：WB-Tr, Human, A-549, Calu3, NCI-H1299 cells.
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Evidence for the link between defective autophagy and inflammation in peripheral blood mononuclear cells of type 2 diabetic patients.
Alizadeh S, Mazloom H, Sadeghi A, Emamgholipour S, Golestani A, Noorbakhsh F, Khoshniatnikoo M, Meshkani R.
Journal of Physiology and Biochemistry 2018 Apr; [Epub].
Application：WB-Ce, Human, Peripheral blood mononuclear cells.
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Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.
The TH, Feltkamp TE.
Immunology 1970 Jun; 18(6):865.
Application：Conjugation, Fluorescein isothiocyanate (FITC).
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Hsa_circ_0016760 exacerbates the malignant development of non‑small cell lung cancer by sponging miR‑145‑5p/FGF5.
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