Sheep Anti-Mouse IgG (H&L) secondary antibody (Rhodamine)
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Specification
Product Description
Sheep anti-mouse IgG recognizes mouse IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Rhodamine.
Immunogen
Mouse IgG whole molecule
Host
Sheep
Reactivity
Mouse
Form
Lyophilized
Conjugation
Rhodamine (TRITC)
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation Note
2.8 moles TRITC per mole of sheep IgG
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
Lyophilized from 10 mM potassium phosphate, 75 mM NaCl, 50% (v/v) glycerol, pH 7.2 (0.01% (w/v) sodium azide, 10 mg/mL BSA (ImmunoglobulLyophilized from and Protease free))
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Flow Cytometry
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Publication Reference
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Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies.
J A Titus, R Haugland, S O Sharrow, D M Segal.
Journal of Immunological Methods 1982 Apr; 50(2):193.
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Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies.
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