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Last updated: 2020/8/2
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Rabbit Anti-Mouse IgG (H&L) secondary antibody (Fluorescein) 

  • Catalog # : PAB10763
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  • Specification
  • Product Description:
  • Rabbit anti-mouse IgG recognizes mouse IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Fluorescein.
  • Immunogen:
  • Mouse IgG whole molecule
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Specificity:
  • Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, mouse IgG and mouse serum.
  • Form:
  • Lyophilized
  • Conjugation:
  • FITC
  • Purification:
  • This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Isotype:
  • IgG
  • Conjugation Note:
  • 2.79 moles Fluorescein (FITC) per mole of IgG
  • Recommend Usage:
  • Flow Cytometry
    Fluorescence-linked immunosorbent assay (1:10000-1:50000)
    Immunofluorescence (1:1000-1:5000)
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% sodium azide).
  • Storage Instruction:
  • Store at 4°C on dry atmosphere prior to restoration.
    After reconstitution with 1.0 mL deionized water (or equivalent), store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Fluorescence-linked Immunosorbent Assay
  • Immunofluorescence
  • Flow Cytometry
  • Application Image
  • Western Blot
  • Fluorescence-linked Immunosorbent Assay
  • Immunofluorescence
  • Flow Cytometry
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