Rabbit Anti-Mouse IgG (H&L) secondary antibody (Fluorescein)
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Specification
Product Description
Rabbit anti-mouse IgG recognizes mouse IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Fluorescein.
Immunogen
Mouse IgG whole molecule
Host
Rabbit
Reactivity
Mouse
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, mouse IgG and mouse serum.
Form
Lyophilized
Conjugation
FITC
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Isotype
IgG
Conjugation Note
2.79 moles Fluorescein (FITC) per mole of IgG
Recommend Usage
Flow Cytometry
Fluorescence-linked immunosorbent assay (1:10000-1:50000)
Immunofluorescence (1:1000-1:5000)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% sodium azide).
Storage Instruction
Store at 4°C on dry atmosphere prior to restoration.
After reconstitution with 1.0 mL deionized water (or equivalent), store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunofluorescence
Flow Cytometry
Fluorescence-linked Immunosorbent Assay
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Publication Reference
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Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.
The TH, Feltkamp TE.
Immunology 1970 Jun; 18(6):865.
Application：Conjugation, Fluorescein isothiocyanate (FITC).
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Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.
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